Novel assay format for total anti-adeno-associated virus antibody detection with low capsid consumption and built-in specificity control
Aim: To develop an assay format for detection of total anti-adeno-associated virus 2 (AAV2) antibodies with low capsid material consumption. Methods: An immune complex (IC) assay format was developed. The format is based on the formation of ICs in solution and their subsequent detection using an anti-AAV2 antibody for capture and an antibody against the study species IgG for detection. Results: The feasibility of the IC assay for detection of preexisting and treatment-emergent anti-AAV2 antibodies was demonstrated in cynomolgus monkey and human serum samples, including samples from a preclinical study with AAV2-based therapies. Conclusion: The presented IC assay is an easy-to-perform total anti-AAV2 antibody assay that requires a small amount of unlabeled capsid material and provides an intrinsic specificity control.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Bioanalysis - (2024) vom: 18. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wessels, Uwe [VerfasserIn] |
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| Links: |
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| Themen: |
Gene therapy |
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| Anmerkungen: |
Date Revised 18.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.4155/bio-2023-0254 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369874625 |
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| 245 | 1 | 0 | |a Novel assay format for total anti-adeno-associated virus antibody detection with low capsid consumption and built-in specificity control |
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| 500 | |a Date Revised 18.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Aim: To develop an assay format for detection of total anti-adeno-associated virus 2 (AAV2) antibodies with low capsid material consumption. Methods: An immune complex (IC) assay format was developed. The format is based on the formation of ICs in solution and their subsequent detection using an anti-AAV2 antibody for capture and an antibody against the study species IgG for detection. Results: The feasibility of the IC assay for detection of preexisting and treatment-emergent anti-AAV2 antibodies was demonstrated in cynomolgus monkey and human serum samples, including samples from a preclinical study with AAV2-based therapies. Conclusion: The presented IC assay is an easy-to-perform total anti-AAV2 antibody assay that requires a small amount of unlabeled capsid material and provides an intrinsic specificity control | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Review | |
| 650 | 4 | |a gene therapy | |
| 650 | 4 | |a immune complex assay | |
| 650 | 4 | |a immunogenicity | |
| 650 | 4 | |a total anti-AAV antibody assay | |
| 700 | 1 | |a Neff, Florian |e verfasserin |4 aut | |
| 700 | 1 | |a Fakhiri, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Mayer, Klaus |e verfasserin |4 aut | |
| 700 | 1 | |a Brinkmann, Ulrich |e verfasserin |4 aut | |
| 700 | 1 | |a Stubenrauch, Kay |e verfasserin |4 aut | |
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