Human Bronchial Epithelial Cell Transcriptome Changes in Response to Serum from Patients with Different Status of Inflammation
© 2024. The Author(s)..
PURPOSE: To investigate the transcriptome of human bronchial epithelial cells (HBEC) in response to serum from patients with different degrees of inflammation.
METHODS: Serum from 19 COVID-19 patients obtained from the Hannover Unified Biobank was used. At the time of sampling, 5 patients had a WHO Clinical Progression Scale (WHO-CPS) score of 9 (severe illness). The remaining 14 patients had a WHO-CPS of below 9 (range 1-7), and lower illness. Multiplex immunoassay was used to assess serum inflammatory markers. The culture medium of HBEC was supplemented with 2% of the patient's serum, and the cells were cultured at 37 °C, 5% CO2 for 18 h. Subsequently, cellular RNA was used for RNA-Seq.
RESULTS: Patients with scores below 9 had significantly lower albumin and serum levels of E-selectin, IL-8, and MCP-1 than patients with scores of 9. Principal component analysis based on 500 "core genes" of RNA-seq segregated cells into two subsets: exposed to serum from 4 (I) and 15 (II) patients. Cells from a subset (I) treated with serum from 4 patients with a score of 9 showed 5566 differentially expressed genes of which 2793 were up- and 2773 downregulated in comparison with cells of subset II treated with serum from 14 patients with scores between 1 and 7 and one with score = 9. In subset I cells, a higher expression of TLR4 and CXCL8 but a lower CDH1, ACE2, and HMOX1, and greater effects on genes involved in metabolic regulation, cytoskeletal organization, and kinase activity pathways were observed.
CONCLUSION: This simple model could be useful to characterize patient serum and epithelial cell properties.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:202 |
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Enthalten in: |
Lung - 202(2024), 2 vom: 06. Apr., Seite 157-170 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Sivaraman, Kokilavani [VerfasserIn] |
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Links: |
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Themen: |
Acute lung inflammation |
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Anmerkungen: |
Date Completed 15.04.2024 Date Revised 25.04.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1007/s00408-024-00679-1 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM369842111 |
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520 | |a © 2024. The Author(s). | ||
520 | |a PURPOSE: To investigate the transcriptome of human bronchial epithelial cells (HBEC) in response to serum from patients with different degrees of inflammation | ||
520 | |a METHODS: Serum from 19 COVID-19 patients obtained from the Hannover Unified Biobank was used. At the time of sampling, 5 patients had a WHO Clinical Progression Scale (WHO-CPS) score of 9 (severe illness). The remaining 14 patients had a WHO-CPS of below 9 (range 1-7), and lower illness. Multiplex immunoassay was used to assess serum inflammatory markers. The culture medium of HBEC was supplemented with 2% of the patient's serum, and the cells were cultured at 37 °C, 5% CO2 for 18 h. Subsequently, cellular RNA was used for RNA-Seq | ||
520 | |a RESULTS: Patients with scores below 9 had significantly lower albumin and serum levels of E-selectin, IL-8, and MCP-1 than patients with scores of 9. Principal component analysis based on 500 "core genes" of RNA-seq segregated cells into two subsets: exposed to serum from 4 (I) and 15 (II) patients. Cells from a subset (I) treated with serum from 4 patients with a score of 9 showed 5566 differentially expressed genes of which 2793 were up- and 2773 downregulated in comparison with cells of subset II treated with serum from 14 patients with scores between 1 and 7 and one with score = 9. In subset I cells, a higher expression of TLR4 and CXCL8 but a lower CDH1, ACE2, and HMOX1, and greater effects on genes involved in metabolic regulation, cytoskeletal organization, and kinase activity pathways were observed | ||
520 | |a CONCLUSION: This simple model could be useful to characterize patient serum and epithelial cell properties | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Acute lung inflammation | |
650 | 4 | |a Chemokine/cytokine profile | |
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700 | 1 | |a Martinez-Delgado, Beatriz |e verfasserin |4 aut | |
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700 | 1 | |a Büttner, Manuela |e verfasserin |4 aut | |
700 | 1 | |a Illig, Thomas |e verfasserin |4 aut | |
700 | 1 | |a Volland, Sonja |e verfasserin |4 aut | |
700 | 1 | |a Gomez-Mariano, Gema |e verfasserin |4 aut | |
700 | 1 | |a Jedicke, Nils |e verfasserin |4 aut | |
700 | 1 | |a Yevsa, Tetyana |e verfasserin |4 aut | |
700 | 1 | |a Welte, Tobias |e verfasserin |4 aut | |
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700 | 1 | |a Janciauskiene, Sabina |e verfasserin |4 aut | |
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