A monodispersed metal-complexing peptide-based polymer for mass cytometry enabling spectral applications
Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved..
We report the synthesis of a novel class of metal-complexing peptide-based polymers, which we name HyperMAPs (Hyper-loaded MetAl-complexed Polymers). The controlled solid-phase synthesis of HyperMAPs' scaffold peptide provides our polymer with a well-defined molecular structure that allows for an accurate on-design assembly of a wide variety of metals. The peptide-scaffold features a handle for direct conjugation to antibodies or any other biomolecules by means of a thiol-maleimide-click or aldehyde-oxime reaction, a fluorogenic moiety for biomolecule conjugation tracking, and a well-defined number of functional groups for direct incorporation of metal-chelator complexes. Since metal-chelator complexes are prepared in a separate reaction prior to incorporation to the peptide scaffold, polymers can be designed to contain specific ratios of metal isotopes, providing each polymer with a unique CyTOF spectral fingerprint. We demonstrate the complexing of 21 different metals using two different chelators and provide evidence of the application of HyperMAPs on a 13 parameter CyTOF panel and compare its performance to monoisotopic metal-conjugated antibodies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:81 |
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| Enthalten in: |
New biotechnology - 81(2024) vom: 25. Apr., Seite 33-42 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Verhoeff, Jan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.04.2024 Date Revised 12.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.nbt.2024.03.002 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a We report the synthesis of a novel class of metal-complexing peptide-based polymers, which we name HyperMAPs (Hyper-loaded MetAl-complexed Polymers). The controlled solid-phase synthesis of HyperMAPs' scaffold peptide provides our polymer with a well-defined molecular structure that allows for an accurate on-design assembly of a wide variety of metals. The peptide-scaffold features a handle for direct conjugation to antibodies or any other biomolecules by means of a thiol-maleimide-click or aldehyde-oxime reaction, a fluorogenic moiety for biomolecule conjugation tracking, and a well-defined number of functional groups for direct incorporation of metal-chelator complexes. Since metal-chelator complexes are prepared in a separate reaction prior to incorporation to the peptide scaffold, polymers can be designed to contain specific ratios of metal isotopes, providing each polymer with a unique CyTOF spectral fingerprint. We demonstrate the complexing of 21 different metals using two different chelators and provide evidence of the application of HyperMAPs on a 13 parameter CyTOF panel and compare its performance to monoisotopic metal-conjugated antibodies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Coordination chemistry | |
| 650 | 4 | |a Mass cytometry | |
| 650 | 4 | |a Polymer | |
| 650 | 4 | |a Rare earth metals | |
| 650 | 4 | |a Spectral cytometry | |
| 650 | 7 | |a Polymers |2 NLM | |
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| 650 | 7 | |a Sulfhydryl Compounds |2 NLM | |
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| 650 | 7 | |a Antibodies |2 NLM | |
| 650 | 7 | |a Maleimides |2 NLM | |
| 700 | 1 | |a van Asten, Saskia |e verfasserin |4 aut | |
| 700 | 1 | |a Kuijper, Lisan |e verfasserin |4 aut | |
| 700 | 1 | |a van den Braber, Marlous |e verfasserin |4 aut | |
| 700 | 1 | |a Amstalden-van Hove, Erika |e verfasserin |4 aut | |
| 700 | 1 | |a Haselberg, Rob |e verfasserin |4 aut | |
| 700 | 1 | |a Kalay, Hakan |e verfasserin |4 aut | |
| 700 | 1 | |a Garcia-Vallejo, Juan J |e verfasserin |4 aut | |
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