Influence of storage solution, temperature, assay time and concentration on RT-qPCR nucleic acid detection for SARS-CoV-2 detection of SARS-CoV-2 by the RT-qPCR
Copyright © 2024. Published by Elsevier Inc..
Real-time reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is an important method for the early diagnosis of coronavirus disease 2019 (COVID-19). This study investigated the effects of storage solution, temperature and detection time on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection by RT-qPCR. Various concentrations of SARS-CoV-2 were added to inactive and non-inactive storage solution and the viral suspensions were stored at various temperatures (room temperature, 4, -20 and -80 °C). Then, at five different detection time points, the Ct values were determined by RT-qPCR. Active and inactive storage solutions and storage temperature have a great impact on the detection of N gene of SARS-CoV-2 at different concentration corridors but have little impact on the ORF gene. The storage time has a greater impact on the N gene and ORF gene at high concentrations but has no effect on the two genes at low concentrations. In conclusion, storage temperature, storage time and storage status (inactivated, non-inactivated) have no effect on the nucleic acid detection of SARS-CoV-2 at the same concentration. For different concentrations of SARS-CoV-2, the detection of N gene is mainly affected.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:707 |
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| Enthalten in: |
Biochemical and biophysical research communications - 707(2024) vom: 07. Apr., Seite 149726 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Su, Lingxuan [VerfasserIn] |
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| Links: |
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| Themen: |
Detection time |
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| Anmerkungen: |
Date Completed 05.04.2024 Date Revised 05.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbrc.2024.149726 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024. Published by Elsevier Inc. | ||
| 520 | |a Real-time reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is an important method for the early diagnosis of coronavirus disease 2019 (COVID-19). This study investigated the effects of storage solution, temperature and detection time on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection by RT-qPCR. Various concentrations of SARS-CoV-2 were added to inactive and non-inactive storage solution and the viral suspensions were stored at various temperatures (room temperature, 4, -20 and -80 °C). Then, at five different detection time points, the Ct values were determined by RT-qPCR. Active and inactive storage solutions and storage temperature have a great impact on the detection of N gene of SARS-CoV-2 at different concentration corridors but have little impact on the ORF gene. The storage time has a greater impact on the N gene and ORF gene at high concentrations but has no effect on the two genes at low concentrations. In conclusion, storage temperature, storage time and storage status (inactivated, non-inactivated) have no effect on the nucleic acid detection of SARS-CoV-2 at the same concentration. For different concentrations of SARS-CoV-2, the detection of N gene is mainly affected | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Detection time | |
| 650 | 4 | |a RT-qPCR | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a Storage temperature | |
| 650 | 4 | |a Viral storage solution | |
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| 700 | 1 | |a Lou, Yihan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jiaxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Haiyan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Biaofeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Guangshang |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yanjun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Keda |e verfasserin |4 aut | |
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