Carbon dots and covalent organic frameworks based FRET immunosensor for sensitive detection of Escherichia coli O157:H7
Copyright © 2024. Published by Elsevier Ltd..
The combination of carbon dots (CDs) with covalent organic frameworks (COFs) was used to design an innovative sensor based on fluorescence resonance energy transfer (FRET) for the detection of Escherichia coli O157:H7 (E. coli O157:H7) in food samples. Carbon dots were used as fluorescence donors, covalent organic frameworks as fluorescence acceptors. The antibody (Ab) specific to E. coli O157:H7 was used to form a CD-Ab-COF immunosensor by linking CDs and COFs. The antibody was specifically bound with E. coli O157:H7, which caused the connection between CDs and COFs to be interrupted, and the carbon dots exhibited fluorescence restoration. The sensor exhibited a linear detection range spanning from 0 to 106 CFU/mL, with the limit of detection (LOD) of 7 CFU/mL. The analytical performance of the developed immunosensor was evaluated using spiked food samples with different concentrations of E. coli O157:H7, validating the capability of assessing risks in food testing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:447 |
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| Enthalten in: |
Food chemistry - 447(2024) vom: 30. Apr., Seite 138663 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Sunli [VerfasserIn] |
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| Links: |
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| Themen: |
7440-44-0 |
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| Anmerkungen: |
Date Completed 10.04.2024 Date Revised 10.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2024.138663 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024. Published by Elsevier Ltd. | ||
| 520 | |a The combination of carbon dots (CDs) with covalent organic frameworks (COFs) was used to design an innovative sensor based on fluorescence resonance energy transfer (FRET) for the detection of Escherichia coli O157:H7 (E. coli O157:H7) in food samples. Carbon dots were used as fluorescence donors, covalent organic frameworks as fluorescence acceptors. The antibody (Ab) specific to E. coli O157:H7 was used to form a CD-Ab-COF immunosensor by linking CDs and COFs. The antibody was specifically bound with E. coli O157:H7, which caused the connection between CDs and COFs to be interrupted, and the carbon dots exhibited fluorescence restoration. The sensor exhibited a linear detection range spanning from 0 to 106 CFU/mL, with the limit of detection (LOD) of 7 CFU/mL. The analytical performance of the developed immunosensor was evaluated using spiked food samples with different concentrations of E. coli O157:H7, validating the capability of assessing risks in food testing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Carbon dots | |
| 650 | 4 | |a Covalent organic frameworks | |
| 650 | 4 | |a Escherichia coli O157:H7 | |
| 650 | 4 | |a Fluorescence resonance energy transfer | |
| 650 | 4 | |a Immunosensor | |
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| 700 | 1 | |a Hu, Xuetao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wenting |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Ziang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xinai |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xiaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhihua |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Xiaobo |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Jiyong |e verfasserin |4 aut | |
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