Investigation into potential allergenicity of DBD plasma-treated casein digestion products based on immunoglobulin E linear epitopes and the sensitized-cell model
Copyright © 2024 Elsevier Ltd. All rights reserved..
The study aimed to investigate the allergenicity change in casein treated with dielectric barrier discharge (DBD) plasma during in vitro simulated digestion, focusing on the immunoglobulin E (IgE) linear epitopes and utilizing a sensitized-cell model. Results indicated that prior treatment with DBD plasma treatment (4 min) before simulated digestion led to a 10.5% reduction in the IgE-binding capacity of casein digestion products. Moreover, the release of biologically active substances induced from KU812 cells, including β-HEX release rate, human histamine, IL-4, IL-6, and TNF-α, decreased by 2.1, 28.1, 20.6, 11.6, and 17.3%, respectively. Through a combined analysis of LC-MS/MS and immunoinformatics tools, it was revealed that DBD plasma treatment promoted the degradation of the IgE linear epitopes of casein during digestion, particularly those located in the α-helix region of αs1-CN and αs2-CN. These findings suggest that DBD plasma treatment prior to digestion may alleviate casein allergic reactions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:447 |
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| Enthalten in: |
Food chemistry - 447(2024) vom: 30. Apr., Seite 138940 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Yongniu [VerfasserIn] |
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| Links: |
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| Themen: |
37341-29-0 |
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| Anmerkungen: |
Date Completed 10.04.2024 Date Revised 10.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2024.138940 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369742338 |
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| 520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The study aimed to investigate the allergenicity change in casein treated with dielectric barrier discharge (DBD) plasma during in vitro simulated digestion, focusing on the immunoglobulin E (IgE) linear epitopes and utilizing a sensitized-cell model. Results indicated that prior treatment with DBD plasma treatment (4 min) before simulated digestion led to a 10.5% reduction in the IgE-binding capacity of casein digestion products. Moreover, the release of biologically active substances induced from KU812 cells, including β-HEX release rate, human histamine, IL-4, IL-6, and TNF-α, decreased by 2.1, 28.1, 20.6, 11.6, and 17.3%, respectively. Through a combined analysis of LC-MS/MS and immunoinformatics tools, it was revealed that DBD plasma treatment promoted the degradation of the IgE linear epitopes of casein during digestion, particularly those located in the α-helix region of αs1-CN and αs2-CN. These findings suggest that DBD plasma treatment prior to digestion may alleviate casein allergic reactions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Allergenicity | |
| 650 | 4 | |a Bioinformatics analysis | |
| 650 | 4 | |a Casein | |
| 650 | 4 | |a DBD plasma treatment | |
| 650 | 4 | |a In vitro digestion | |
| 650 | 4 | |a Structure | |
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| 650 | 7 | |a Immunoglobulin E |2 NLM | |
| 650 | 7 | |a 37341-29-0 |2 NLM | |
| 650 | 7 | |a Allergens |2 NLM | |
| 650 | 7 | |a Caseins |2 NLM | |
| 700 | 1 | |a Ren, Er-Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Lyu, Shijun |e verfasserin |4 aut | |
| 700 | 1 | |a Gai, Lili |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Siyu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Fuge |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Debao |e verfasserin |4 aut | |
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