In-process quality control in foot-and-mouth disease vaccine production by detection of viral non-structural proteins using chemiluminescence dot blot assay
Copyright © 2024 Elsevier B.V. All rights reserved..
Foot-and-mouth disease (FMD) is a contagious viral disease of cloven-footed animals. Immunization with inactivated virus vaccine is effective to control the disease. Six-monthly vaccination regimen in endemic regions has proven to be effective. To enable the differentiation of infected animals from those vaccinated, non-structural proteins (NSPs) are excluded during vaccine production. While the antibodies to structural proteins (SPs) could be observed both in vaccinated and infected animals, NSP antibodies are detectable only in natural infection. Quality control assays that detect NSPs in vaccine antigen preparations, are thus vital in the FMD vaccine manufacturing process. In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:326 |
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| Enthalten in: |
Journal of virological methods - 326(2024) vom: 20. Apr., Seite 114906 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jabeen, Uzma [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 09.04.2024 Date Revised 09.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jviromet.2024.114906 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369688058 |
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| 520 | |a Foot-and-mouth disease (FMD) is a contagious viral disease of cloven-footed animals. Immunization with inactivated virus vaccine is effective to control the disease. Six-monthly vaccination regimen in endemic regions has proven to be effective. To enable the differentiation of infected animals from those vaccinated, non-structural proteins (NSPs) are excluded during vaccine production. While the antibodies to structural proteins (SPs) could be observed both in vaccinated and infected animals, NSP antibodies are detectable only in natural infection. Quality control assays that detect NSPs in vaccine antigen preparations, are thus vital in the FMD vaccine manufacturing process. In this study, we designed a chemiluminescence dot blot assay to detect the 3A and 3B NSPs of FMDV. It is sensitive enough to detect up to 20 ng of the NSP, and exhibited specificity as it does not react with the viral SPs. This cost-effective assay holds promise in quality control assessment in FMD vaccine manufacturing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chemiluminescence dot blot assay | |
| 650 | 4 | |a Foot-and-mouth disease vaccine | |
| 650 | 4 | |a Non-structural proteins | |
| 650 | 4 | |a Vaccine quality | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Viral Vaccines |2 NLM | |
| 650 | 7 | |a Viral Nonstructural Proteins |2 NLM | |
| 700 | 1 | |a Bisht, Kailash Singh |e verfasserin |4 aut | |
| 700 | 1 | |a Ranjitha, Huildore Bommanna |e verfasserin |4 aut | |
| 700 | 1 | |a Hosamani, Madhusudan |e verfasserin |4 aut | |
| 700 | 1 | |a Sreenivasa, Beeragere Parameshwaraiah |e verfasserin |4 aut | |
| 700 | 1 | |a Kulkarni, Pratik M |e verfasserin |4 aut | |
| 700 | 1 | |a Nidhi, Dombesara Chandrashekar |e verfasserin |4 aut | |
| 700 | 1 | |a Amulya, Rajegowdanadoddi Lakshmana |e verfasserin |4 aut | |
| 700 | 1 | |a Bhanuprakash, Veerakyathappa |e verfasserin |4 aut | |
| 700 | 1 | |a Dechamma, Hosur Joyappa |e verfasserin |4 aut | |
| 700 | 1 | |a Sanyal, Aniket |e verfasserin |4 aut | |
| 700 | 1 | |a Basagoudanavar, Suresh H |e verfasserin |4 aut | |
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