Short- and Long-Term Storage of Non-Domesticated European Mouflon (Ovis aries musimon) Cumulus-Oocyte Complexes Recovered in Field Conditions
Reproductive biotechnologies can be used as a supporting tool, through gamete conservation and in vitro embryo production, in the preservation of invaluable and irreplaceable animal genetic resources. In the present study, immature mouflon cumulus-oocyte complexes (COCs) collected from ovariectomized female ovaries underwent short- or long-term conservation (24 h maintained in Earle's/Hank's (EH) medium or vitrification) under field conditions and afterwards transported to the laboratory where they were cultured for in vitro maturation (IVM) and assessed for oocyte meiotic competence and bioenergetic-oxidative status. Utilization of both storage techniques led to COC morphology preservation, as well as cumulus expansion and oocyte meiotic resumption after the IVM procedure. Quantitative bioenergetic-oxidative parameters were reduced in vitrified oocytes compared with EH ones. Immature COC storage needs to be optimized in both domesticated and non-domesticated sheep as a part of the strategy to avoid the loss of valuable genotypes of these animal species.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Animals : an open access journal from MDPI - 14(2024), 5 vom: 05. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Temerario, Letizia [VerfasserIn] |
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| Links: |
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| Themen: |
Bioenergetic–oxidative status |
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| Anmerkungen: |
Date Revised 15.03.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/ani14050807 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369629078 |
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| 520 | |a Reproductive biotechnologies can be used as a supporting tool, through gamete conservation and in vitro embryo production, in the preservation of invaluable and irreplaceable animal genetic resources. In the present study, immature mouflon cumulus-oocyte complexes (COCs) collected from ovariectomized female ovaries underwent short- or long-term conservation (24 h maintained in Earle's/Hank's (EH) medium or vitrification) under field conditions and afterwards transported to the laboratory where they were cultured for in vitro maturation (IVM) and assessed for oocyte meiotic competence and bioenergetic-oxidative status. Utilization of both storage techniques led to COC morphology preservation, as well as cumulus expansion and oocyte meiotic resumption after the IVM procedure. Quantitative bioenergetic-oxidative parameters were reduced in vitrified oocytes compared with EH ones. Immature COC storage needs to be optimized in both domesticated and non-domesticated sheep as a part of the strategy to avoid the loss of valuable genotypes of these animal species | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a bioenergetic–oxidative status | |
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| 650 | 4 | |a vitrification | |
| 700 | 1 | |a Cicirelli, Vincenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Martino, Nicola Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Carbonari, Alice |e verfasserin |4 aut | |
| 700 | 1 | |a Burgio, Matteo |e verfasserin |4 aut | |
| 700 | 1 | |a Frattina, Lorenza |e verfasserin |4 aut | |
| 700 | 1 | |a Lacalandra, Giovanni Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Rizzo, Annalisa |e verfasserin |4 aut | |
| 700 | 1 | |a Dell'Aquila, Maria Elena |e verfasserin |4 aut | |
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