Transposition of HOPPLA in siRNA-deficient plants suggests a limited effect of the environment on retrotransposon mobility in Brachypodium distachyon
Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication..
Long terminal repeat retrotransposons (LTR-RTs) are powerful mutagens regarded as a major source of genetic novelty and important drivers of evolution. Yet, the uncontrolled and potentially selfish proliferation of LTR-RTs can lead to deleterious mutations and genome instability, with large fitness costs for their host. While population genomics data suggest that an ongoing LTR-RT mobility is common in many species, the understanding of their dual role in evolution is limited. Here, we harness the genetic diversity of 320 sequenced natural accessions of the Mediterranean grass Brachypodium distachyon to characterize how genetic and environmental factors influence plant LTR-RT dynamics in the wild. When combining a coverage-based approach to estimate global LTR-RT copy number variations with mobilome-sequencing of nine accessions exposed to eight different stresses, we find little evidence for a major role of environmental factors in LTR-RT accumulations in B. distachyon natural accessions. Instead, we show that loss of RNA polymerase IV (Pol IV), which mediates RNA-directed DNA methylation in plants, results in high transcriptional and transpositional activities of RLC_BdisC024 (HOPPLA) LTR-RT family elements, and that these effects are not stress-specific. This work supports findings indicating an ongoing mobility in B. distachyon and reveals that host RNA-directed DNA methylation rather than environmental factors controls their mobility in this wild grass model.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
PLoS genetics - 20(2024), 3 vom: 19. März, Seite e1011200 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Thieme, Michael [VerfasserIn] |
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Date Completed 25.03.2024 Date Revised 25.03.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1371/journal.pgen.1011200 |
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| 245 | 1 | 0 | |a Transposition of HOPPLA in siRNA-deficient plants suggests a limited effect of the environment on retrotransposon mobility in Brachypodium distachyon |
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| 520 | |a Long terminal repeat retrotransposons (LTR-RTs) are powerful mutagens regarded as a major source of genetic novelty and important drivers of evolution. Yet, the uncontrolled and potentially selfish proliferation of LTR-RTs can lead to deleterious mutations and genome instability, with large fitness costs for their host. While population genomics data suggest that an ongoing LTR-RT mobility is common in many species, the understanding of their dual role in evolution is limited. Here, we harness the genetic diversity of 320 sequenced natural accessions of the Mediterranean grass Brachypodium distachyon to characterize how genetic and environmental factors influence plant LTR-RT dynamics in the wild. When combining a coverage-based approach to estimate global LTR-RT copy number variations with mobilome-sequencing of nine accessions exposed to eight different stresses, we find little evidence for a major role of environmental factors in LTR-RT accumulations in B. distachyon natural accessions. Instead, we show that loss of RNA polymerase IV (Pol IV), which mediates RNA-directed DNA methylation in plants, results in high transcriptional and transpositional activities of RLC_BdisC024 (HOPPLA) LTR-RT family elements, and that these effects are not stress-specific. This work supports findings indicating an ongoing mobility in B. distachyon and reveals that host RNA-directed DNA methylation rather than environmental factors controls their mobility in this wild grass model | ||
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| 700 | 1 | |a Minadakis, Nikolaos |e verfasserin |4 aut | |
| 700 | 1 | |a Himber, Christophe |e verfasserin |4 aut | |
| 700 | 1 | |a Keller, Bettina |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Wenbo |e verfasserin |4 aut | |
| 700 | 1 | |a Rutowicz, Kinga |e verfasserin |4 aut | |
| 700 | 1 | |a Matteoli, Calvin |e verfasserin |4 aut | |
| 700 | 1 | |a Böhrer, Marcel |e verfasserin |4 aut | |
| 700 | 1 | |a Rymen, Bart |e verfasserin |4 aut | |
| 700 | 1 | |a Laudencia-Chingcuanco, Debbie |e verfasserin |4 aut | |
| 700 | 1 | |a Vogel, John P |e verfasserin |4 aut | |
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| 700 | 1 | |a Stritt, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Blevins, Todd |e verfasserin |4 aut | |
| 700 | 1 | |a Roulin, Anne C |e verfasserin |4 aut | |
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