A novel nucleic acid linker for multi-gene expression enhances plant and animal synthetic biology
© 2024 Society for Experimental Biology and John Wiley & Sons Ltd..
The production of compact vectors for gene stacking is hindered by a lack of effective linkers. Here, we report that a 26-nt nucleic acid linker, NAL1, from the fungus Glarea lozoyensis and its truncated derivatives could connect two genes as a bicistron, enabling independent translation in a maize protoplast transient expression system and human 293 T cells. The optimized 9-nt NAL10 linker was then used to connect four genes driven by a bidirectional promoter; this combination was successfully used to reconstruct the astaxanthin biosynthesis pathway in transgenic maize. The short and efficient nucleic acid linker NAL10 can be widely used in multi-gene expression and synthetic biology in animals and plants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
The Plant journal : for cell and molecular biology - (2024) vom: 12. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ma, Xuhui [VerfasserIn] |
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| Links: |
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| Themen: |
Astaxanthin |
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| Anmerkungen: |
Date Revised 12.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1111/tpj.16714 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a The production of compact vectors for gene stacking is hindered by a lack of effective linkers. Here, we report that a 26-nt nucleic acid linker, NAL1, from the fungus Glarea lozoyensis and its truncated derivatives could connect two genes as a bicistron, enabling independent translation in a maize protoplast transient expression system and human 293 T cells. The optimized 9-nt NAL10 linker was then used to connect four genes driven by a bidirectional promoter; this combination was successfully used to reconstruct the astaxanthin biosynthesis pathway in transgenic maize. The short and efficient nucleic acid linker NAL10 can be widely used in multi-gene expression and synthetic biology in animals and plants | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Miao, Liqing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Suzhen |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Si, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Liwen |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Wenzhu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xiaojin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Junmin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Rumei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Yuquan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoqing |e verfasserin |4 aut | |
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