TP508 Promotes Bone Regeneration on Distraction Osteogenesis via the Activation of Wnt/β-catenin Signaling Pathway
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net..
INTRODUCTION: TP508 is a thrombin peptide that participates in the inflammatory response and wound healing. Its role in the molecular mechanism of distraction osteogenesis remains unclear. This study established a tibia distraction osteogenesis (DO) model in rats and investigated the role and mechanism of TP508 in bone regeneration during DO.
METHOD: Micro-computed tomography (Micro-CT) and hematoxylin-eosin (HE) staining were used to track osteogenesis. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to measure the expression of osteoblast-related factors, Wnt/β- catenin signaling-related proteins and genes. Immunohistochemistry was used to measure the expression of β-catenin in the cytoplasm and nucleus. TP508 accelerated bone regeneration increased the expression of the osteoblast-related factors Alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN).
RESULTS: After the Wnt signaling was inhibited by LGK974, the expression of osteoblast-related factors was downregulated, leading to a decrease in bone regeneration ability. More importantly, TP508 upregulated β-catenin and its target CYCLIN-D1 and could reverse the decreased osteogenic ability caused by LGK974.
CONCLUSION: In conclusion, TP508 promotes bone regeneration in DO by activating the Wnt/β- catenin signaling pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Current pharmaceutical biotechnology - (2024) vom: 11. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Kehan [VerfasserIn] |
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| Links: |
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| Themen: |
Distraction osteogenesis |
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| Anmerkungen: |
Date Revised 12.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.2174/0113892010289575240306033011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369582527 |
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| 100 | 1 | |a Li, Kehan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a TP508 Promotes Bone Regeneration on Distraction Osteogenesis via the Activation of Wnt/β-catenin Signaling Pathway |
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| 500 | |a Date Revised 12.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net. | ||
| 520 | |a INTRODUCTION: TP508 is a thrombin peptide that participates in the inflammatory response and wound healing. Its role in the molecular mechanism of distraction osteogenesis remains unclear. This study established a tibia distraction osteogenesis (DO) model in rats and investigated the role and mechanism of TP508 in bone regeneration during DO | ||
| 520 | |a METHOD: Micro-computed tomography (Micro-CT) and hematoxylin-eosin (HE) staining were used to track osteogenesis. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to measure the expression of osteoblast-related factors, Wnt/β- catenin signaling-related proteins and genes. Immunohistochemistry was used to measure the expression of β-catenin in the cytoplasm and nucleus. TP508 accelerated bone regeneration increased the expression of the osteoblast-related factors Alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN) | ||
| 520 | |a RESULTS: After the Wnt signaling was inhibited by LGK974, the expression of osteoblast-related factors was downregulated, leading to a decrease in bone regeneration ability. More importantly, TP508 upregulated β-catenin and its target CYCLIN-D1 and could reverse the decreased osteogenic ability caused by LGK974 | ||
| 520 | |a CONCLUSION: In conclusion, TP508 promotes bone regeneration in DO by activating the Wnt/β- catenin signaling pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a TP508 | |
| 650 | 4 | |a Wnt signalling | |
| 650 | 4 | |a distraction osteogenesis | |
| 650 | 4 | |a molecular mechanism | |
| 650 | 4 | |a thrombin peptide | |
| 650 | 4 | |a tissue engineering | |
| 700 | 1 | |a Liu, Linan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jingyi |e verfasserin |4 aut | |
| 700 | 1 | |a Liao, Chenyu |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jian |e verfasserin |4 aut | |
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