Monoclonal neutralizing antibodies against SARS-COV-2 S protein
AJTR Copyright © 2024..
Novel coronavirus pneumonia, also known as coronavirus disease 2019 (COVID-19), is caused by sub-severe acute respiratory syndrome type 2 coronavirus (SARS-CoV-2) infection. The spike (S) protein of SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) receptors widely expressed on the surface of human cells leading to life-threatening respiratory infections. A serious hazard to human health is posed by the lack of particular treatment medications for this virus infection. We advocate the creation of high-affinity antibodies using the receptor binding domain (RBD) of S protein as a specific antigenic epitope to develop a drug that can precisely target therapy COVID-19 because SARS-CoV-2 infection of the host cells is dependent on S protein binding to ACE2. Finally, we obtained high-affinity antibodies 14F4HL and 14E3HL that have high affinity with RBD and well-drug-forming properties, suitable for further humanization studies. Thus, monoclonal antibodies that neutralize the S protein were identified in our study, which may provide new insights for the development of COVID-19 therapeutic drugs.
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
---|---|
Enthalten in: |
American journal of translational research - 16(2024), 2 vom: 28., Seite 681-689 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Cheng, Lin-Dong [VerfasserIn] |
---|
Themen: |
ACE2 |
---|
Anmerkungen: |
Date Revised 12.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
---|
Förderinstitution / Projekttitel: |
|
---|
PPN (Katalog-ID): |
NLM369533305 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM369533305 | ||
003 | DE-627 | ||
005 | 20240312234314.0 | ||
007 | tu | ||
008 | 240311s2024 xx ||||| 00| ||eng c | ||
028 | 5 | 2 | |a pubmed24n1324.xml |
035 | |a (DE-627)NLM369533305 | ||
035 | |a (NLM)38463597 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Cheng, Lin-Dong |e verfasserin |4 aut | |
245 | 1 | 0 | |a Monoclonal neutralizing antibodies against SARS-COV-2 S protein |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Revised 12.03.2024 | ||
500 | |a published: Electronic-eCollection | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a AJTR Copyright © 2024. | ||
520 | |a Novel coronavirus pneumonia, also known as coronavirus disease 2019 (COVID-19), is caused by sub-severe acute respiratory syndrome type 2 coronavirus (SARS-CoV-2) infection. The spike (S) protein of SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) receptors widely expressed on the surface of human cells leading to life-threatening respiratory infections. A serious hazard to human health is posed by the lack of particular treatment medications for this virus infection. We advocate the creation of high-affinity antibodies using the receptor binding domain (RBD) of S protein as a specific antigenic epitope to develop a drug that can precisely target therapy COVID-19 because SARS-CoV-2 infection of the host cells is dependent on S protein binding to ACE2. Finally, we obtained high-affinity antibodies 14F4HL and 14E3HL that have high affinity with RBD and well-drug-forming properties, suitable for further humanization studies. Thus, monoclonal antibodies that neutralize the S protein were identified in our study, which may provide new insights for the development of COVID-19 therapeutic drugs | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a ACE2 | |
650 | 4 | |a COVID-19 | |
650 | 4 | |a SARS-CoV-2 | |
650 | 4 | |a hybridoma fusion | |
650 | 4 | |a monoclonal antibody | |
700 | 1 | |a Li, Ping |e verfasserin |4 aut | |
700 | 1 | |a Lin, Yan-Chen |e verfasserin |4 aut | |
700 | 1 | |a Hu, Hui-Xiu |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Ying |e verfasserin |4 aut | |
700 | 1 | |a Li, Hou-Feng |e verfasserin |4 aut | |
700 | 1 | |a Huang, Jing |e verfasserin |4 aut | |
700 | 1 | |a Tan, Li |e verfasserin |4 aut | |
700 | 1 | |a Ma, Ning |e verfasserin |4 aut | |
700 | 1 | |a Xia, Deng-Yun |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t American journal of translational research |d 2009 |g 16(2024), 2 vom: 28., Seite 681-689 |w (DE-627)NLM193229463 |x 1943-8141 |7 nnns |
773 | 1 | 8 | |g volume:16 |g year:2024 |g number:2 |g day:28 |g pages:681-689 |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 16 |j 2024 |e 2 |b 28 |h 681-689 |