Detection of Mycobacterium tuberculosis DNA in CD34+ peripheral blood mononuclear cells of adults with tuberculosis infection and disease
Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved..
OBJECTIVES: To investigate whether Mycobacterium tuberculosis (Mtb) DNA is detected in peripheral blood mononuclear cells (PBMC) of subjects with tuberculosis (TB) or TB infection (TBI) living in a low-burden country.
METHODS: We prospectively enrolled 57 patients with TB, 41 subjects with TBI, and 39 controls in Rome, Italy. PBMC were isolated, cluster of differentiation (CD)34+ and CD34- cells were immunomagnetic separated, DNA was extracted, and digital polymerase chain reaction for IS6110 and rpoB sequences was used to detect Mtb DNA in PBMC subsets and unfractionated PBMC.
RESULTS: We detected Mtb DNA at a low copy number in CD34+ cells in 4o f 30 (13%) patients with TB, 2 of 24 (8%) subjects with TBI, and 1 of 24 (4%) controls. Mtb DNA was detected in unfractionated PBMC in 3 of 51 (6%) patients with TB, 2 of 38 (5%) subjects with TBI, and 2 of 36 (6%) controls. In CD34- cells, only 1 of 31 (3%) subjects with TBI tested positive for Mtb DNA.
CONCLUSIONS: Mtb DNA was detected at low frequencies and levels in the PBMC of subjects with TBI and donors with TB living in a low-burden country. In particular, Mtb DNA was detected more frequently in CD34+ cells, supporting the hypothesis that these cells may represent a Mtb niche. This finding informs biological understanding of Mtb pathogenesis and may support the development of a microbial blood biomarker for Mtb infection.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:141S |
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Enthalten in: |
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 141S(2024) vom: 02. Apr., Seite 106999 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Repele, Federica [VerfasserIn] |
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Links: |
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Themen: |
CD34(+) cells |
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Anmerkungen: |
Date Completed 15.04.2024 Date Revised 17.04.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.ijid.2024.106999 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM369481631 |
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245 | 1 | 0 | |a Detection of Mycobacterium tuberculosis DNA in CD34+ peripheral blood mononuclear cells of adults with tuberculosis infection and disease |
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500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
520 | |a OBJECTIVES: To investigate whether Mycobacterium tuberculosis (Mtb) DNA is detected in peripheral blood mononuclear cells (PBMC) of subjects with tuberculosis (TB) or TB infection (TBI) living in a low-burden country | ||
520 | |a METHODS: We prospectively enrolled 57 patients with TB, 41 subjects with TBI, and 39 controls in Rome, Italy. PBMC were isolated, cluster of differentiation (CD)34+ and CD34- cells were immunomagnetic separated, DNA was extracted, and digital polymerase chain reaction for IS6110 and rpoB sequences was used to detect Mtb DNA in PBMC subsets and unfractionated PBMC | ||
520 | |a RESULTS: We detected Mtb DNA at a low copy number in CD34+ cells in 4o f 30 (13%) patients with TB, 2 of 24 (8%) subjects with TBI, and 1 of 24 (4%) controls. Mtb DNA was detected in unfractionated PBMC in 3 of 51 (6%) patients with TB, 2 of 38 (5%) subjects with TBI, and 2 of 36 (6%) controls. In CD34- cells, only 1 of 31 (3%) subjects with TBI tested positive for Mtb DNA | ||
520 | |a CONCLUSIONS: Mtb DNA was detected at low frequencies and levels in the PBMC of subjects with TBI and donors with TB living in a low-burden country. In particular, Mtb DNA was detected more frequently in CD34+ cells, supporting the hypothesis that these cells may represent a Mtb niche. This finding informs biological understanding of Mtb pathogenesis and may support the development of a microbial blood biomarker for Mtb infection | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a CD34(+) cells | |
650 | 4 | |a IGRA | |
650 | 4 | |a Mycobacterium tuberculosis | |
650 | 4 | |a Tuberculosis infection | |
650 | 4 | |a Tuberculosis niche | |
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700 | 1 | |a Navarra, Assunta |e verfasserin |4 aut | |
700 | 1 | |a Farroni, Chiara |e verfasserin |4 aut | |
700 | 1 | |a Salmi, Andrea |e verfasserin |4 aut | |
700 | 1 | |a Cuzzi, Gilda |e verfasserin |4 aut | |
700 | 1 | |a Delogu, Giovanni |e verfasserin |4 aut | |
700 | 1 | |a Gualano, Gina |e verfasserin |4 aut | |
700 | 1 | |a Puro, Vincenzo |e verfasserin |4 aut | |
700 | 1 | |a De Carli, Gabriella |e verfasserin |4 aut | |
700 | 1 | |a Girardi, Enrico |e verfasserin |4 aut | |
700 | 1 | |a Palmieri, Fabrizio |e verfasserin |4 aut | |
700 | 1 | |a Martineau, Adrian R |e verfasserin |4 aut | |
700 | 1 | |a Goletti, Delia |e verfasserin |4 aut | |
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