Light-controllable cell-membrane disturbance for intracellular delivery
Highly polar and charged molecules, such as oligonucleotides, face significant barriers in crossing the cell membrane to access the cytoplasm. To address this problem, we developed a light-triggered twistable tetraphenylethene (TPE) derivative, TPE-C-N, to facilitate the intracellular delivery of charged molecules through an endocytosis-independent pathway. The central double bond of TPE in TPE-C-N is planar in the ground state but becomes twisted in the excited state. Under light irradiation, this planar-to-twisted structural change induces continuous cell membrane disturbances. Such disturbance does not lead to permanent damage to the cell membrane. TPE-C-N significantly enhanced the intracellular delivery of negatively charged molecules under light irradiation when endocytosis was inhibited through low-temperature treatment, confirming the endocytosis-independent nature of this delivery method. We have successfully demonstrated that the TPE-C-N-mediated light-controllable method can efficiently promote the intracellular delivery of charged molecules, such as peptides and oligonucleotides, with molecular weights ranging from 1000 to 5000 Da.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Journal of materials chemistry. B - 12(2024), 17 vom: 01. Mai, Seite 4138-4147 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huo, Wenting [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 01.05.2024 Date Revised 01.05.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1039/d3tb02956e |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369462904 |
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| 520 | |a Highly polar and charged molecules, such as oligonucleotides, face significant barriers in crossing the cell membrane to access the cytoplasm. To address this problem, we developed a light-triggered twistable tetraphenylethene (TPE) derivative, TPE-C-N, to facilitate the intracellular delivery of charged molecules through an endocytosis-independent pathway. The central double bond of TPE in TPE-C-N is planar in the ground state but becomes twisted in the excited state. Under light irradiation, this planar-to-twisted structural change induces continuous cell membrane disturbances. Such disturbance does not lead to permanent damage to the cell membrane. TPE-C-N significantly enhanced the intracellular delivery of negatively charged molecules under light irradiation when endocytosis was inhibited through low-temperature treatment, confirming the endocytosis-independent nature of this delivery method. We have successfully demonstrated that the TPE-C-N-mediated light-controllable method can efficiently promote the intracellular delivery of charged molecules, such as peptides and oligonucleotides, with molecular weights ranging from 1000 to 5000 Da | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Miki, Koji |e verfasserin |4 aut | |
| 700 | 1 | |a Mu, Huiying |e verfasserin |4 aut | |
| 700 | 1 | |a Osawa, Takashi |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaguma, Harumi |e verfasserin |4 aut | |
| 700 | 1 | |a Kasahara, Yuuya |e verfasserin |4 aut | |
| 700 | 1 | |a Obika, Satoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Kawaguchi, Yoshimasa |e verfasserin |4 aut | |
| 700 | 1 | |a Hirose, Hisaaki |e verfasserin |4 aut | |
| 700 | 1 | |a Futaki, Shiroh |e verfasserin |4 aut | |
| 700 | 1 | |a Miyazaki, Yusuke |e verfasserin |4 aut | |
| 700 | 1 | |a Shinoda, Wataru |e verfasserin |4 aut | |
| 700 | 1 | |a Akai, Shuji |e verfasserin |4 aut | |
| 700 | 1 | |a Ohe, Kouichi |e verfasserin |4 aut | |
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