Lowering Entropic Barriers in Triplex DNA Switches Facilitating Biomedical Applications at Physiological pH
© 2024 Wiley‐VCH GmbH..
Triplex DNA switches are attractive allosteric tools for engineering smart nanodevices, but their poor triplex-forming capacity at physiological conditions limited the practical applications. To address this challenge, we proposed a low-entropy barrier design to facilitate triplex formation by introducing a hairpin duplex linker into the triplex motif, and the resulting triplex switch was termed as CTNSds. Compared to the conventional clamp-like triplex switch, CTNSds increased the triplex-forming ratio from 30 % to 91 % at pH 7.4 and stabilized the triple-helix structure in FBS and cell lysate. CTNSds was also less sensitive to free-energy disturbances, such as lengthening linkers or mismatches in the triple-helix stem. The CTNSds design was utilized to reversibly isolate CTCs from whole blood, achieving high capture efficiencies (>86 %) at pH 7.4 and release efficiencies (>80 %) at pH 8.0. Our approach broadens the potential applications of DNA switches-based switchable nanodevices, showing great promise in biosensing and biomedicine.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Angewandte Chemie (International ed. in English) - 63(2024), 19 vom: 06. Apr., Seite e202402123 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lei, Yanli [VerfasserIn] |
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| Links: |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 25.04.2024 Date Revised 25.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/anie.202402123 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Triplex DNA switches are attractive allosteric tools for engineering smart nanodevices, but their poor triplex-forming capacity at physiological conditions limited the practical applications. To address this challenge, we proposed a low-entropy barrier design to facilitate triplex formation by introducing a hairpin duplex linker into the triplex motif, and the resulting triplex switch was termed as CTNSds. Compared to the conventional clamp-like triplex switch, CTNSds increased the triplex-forming ratio from 30 % to 91 % at pH 7.4 and stabilized the triple-helix structure in FBS and cell lysate. CTNSds was also less sensitive to free-energy disturbances, such as lengthening linkers or mismatches in the triple-helix stem. The CTNSds design was utilized to reversibly isolate CTCs from whole blood, achieving high capture efficiencies (>86 %) at pH 7.4 and release efficiencies (>80 %) at pH 8.0. Our approach broadens the potential applications of DNA switches-based switchable nanodevices, showing great promise in biosensing and biomedicine | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Biosensing | |
| 650 | 4 | |a Cell capture and release | |
| 650 | 4 | |a Entropic barriers | |
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| 700 | 1 | |a Ji, Xinyue |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Haiyan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaowen |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Zenghui |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Weiju |e verfasserin |4 aut | |
| 700 | 1 | |a Qing, Zhihe |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Juewen |e verfasserin |4 aut | |
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