Glycyrrhizic acid treatment ameliorates anxiety-like behaviour via GLT1 and Per1/2-dependent pathways
Copyright © 2024 Elsevier B.V. All rights reserved..
ETHNOPHARMACOLOGICAL RELEVANCE: As a traditional Chinese medicinal herb, Glycyrrhiza.
URALENSIS: Fisch. (licorice root, chinese name: Gancao) has a variety of medicinal values and is widely used clinically. Its main active ingredient, glycyrrhizic acid (GA), is believed to have a neuroprotective effect. However, the underlying biological mechanisms of GA on stress-induced anxiety disorders are still unclear.
AIM OF THE STUDY: To investigate the anti-anxiety effect of GA and its underlying mechanism.
METHODS: We selected the anxiety model induced by repeated chronic restraint stress (CRS) for 2 h on each of 7 consecutive days. GA (4, 20, 100 mg/kg) was injected intraperitoneally once daily for 1 week. The potential GA receptors were identified using whole-cell patches and computer-assisted docking of molecules. High-throughput RNA sequencing, adeno-associated virus-mediated gene regulation, Western blotting, and RT-qPCR were used to assess the underlying molecular pathways.
RESULTS: GA alleviate depression-like and anxiety-like behaviors in CRS mice. GA decreased synaptic transmission by facilitating glutamate reuptaking in mPFC. Meanwhile, long-term GA treatment increased the expression of clock genes Per1 and Per2. Suppressing both Per1 and Per2 abolished the anxiolytic effects of GA treatment.
CONCLUSION: Our study suggests that GA may be developed for the treatment of stress-induced anxiety disorders, and its mechanism is related to GLT1 and Per1/2-dependent pathways. This presents a novel approach to discovering potent therapeutic drugs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:328 |
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| Enthalten in: |
Journal of ethnopharmacology - 328(2024) vom: 28. Apr., Seite 118013 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ma, Shanbo [VerfasserIn] |
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| Links: |
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| Themen: |
6FO62043WK |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jep.2024.118013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369428420 |
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| 100 | 1 | |a Ma, Shanbo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Glycyrrhizic acid treatment ameliorates anxiety-like behaviour via GLT1 and Per1/2-dependent pathways |
| 264 | 1 | |c 2024 | |
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| 500 | |a Date Revised 15.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a ETHNOPHARMACOLOGICAL RELEVANCE: As a traditional Chinese medicinal herb, Glycyrrhiza | ||
| 520 | |a URALENSIS: Fisch. (licorice root, chinese name: Gancao) has a variety of medicinal values and is widely used clinically. Its main active ingredient, glycyrrhizic acid (GA), is believed to have a neuroprotective effect. However, the underlying biological mechanisms of GA on stress-induced anxiety disorders are still unclear | ||
| 520 | |a AIM OF THE STUDY: To investigate the anti-anxiety effect of GA and its underlying mechanism | ||
| 520 | |a METHODS: We selected the anxiety model induced by repeated chronic restraint stress (CRS) for 2 h on each of 7 consecutive days. GA (4, 20, 100 mg/kg) was injected intraperitoneally once daily for 1 week. The potential GA receptors were identified using whole-cell patches and computer-assisted docking of molecules. High-throughput RNA sequencing, adeno-associated virus-mediated gene regulation, Western blotting, and RT-qPCR were used to assess the underlying molecular pathways | ||
| 520 | |a RESULTS: GA alleviate depression-like and anxiety-like behaviors in CRS mice. GA decreased synaptic transmission by facilitating glutamate reuptaking in mPFC. Meanwhile, long-term GA treatment increased the expression of clock genes Per1 and Per2. Suppressing both Per1 and Per2 abolished the anxiolytic effects of GA treatment | ||
| 520 | |a CONCLUSION: Our study suggests that GA may be developed for the treatment of stress-induced anxiety disorders, and its mechanism is related to GLT1 and Per1/2-dependent pathways. This presents a novel approach to discovering potent therapeutic drugs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anxiety | |
| 650 | 4 | |a Chronic restraint stress | |
| 650 | 4 | |a Glutamate | |
| 650 | 4 | |a Glycyrrhizic acid | |
| 650 | 4 | |a Per1/2 | |
| 650 | 7 | |a Glycyrrhizic Acid |2 NLM | |
| 650 | 7 | |a 6FO62043WK |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Per1 protein, mouse |2 NLM | |
| 650 | 7 | |a Period Circadian Proteins |2 NLM | |
| 700 | 1 | |a Chong, Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Quan, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Gui, Jiayue |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Long |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Miao, Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Xiaopeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Minggao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Kun |e verfasserin |4 aut | |
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