Adrenal pheochromocytoma impacts three main pathways : cysteine-methionine, pyrimidine, and tyrosine metabolism
Pheochromocytomas and paragangliomas (PPGLs) cause symptoms by altering the circulation levels of catecholamines and peptide hormones. Currently, the diagnosis of PPGLs relies on diagnostic imaging and the detection of catecholamines. In this study, we used ultra-performance liquid chromatography (UPLC)/quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis to identify and measure the perioperative differential metabolites in the plasma of adrenal pheochromocytoma patients. We identified differentially expressed genes by comparing the transcriptomic data of pheochromocytoma with the normal adrenal medulla. Through conducting two steps of metabolomics analysis, we identified 111 differential metabolites between the healthy group and the patient group, among which 53 metabolites were validated. By integrating the information of differential metabolites and differentially expressed genes, we inferred that the cysteine-methionine, pyrimidine, and tyrosine metabolism pathways were the three main metabolic pathways altered by the neoplasm. The analysis of transcription levels revealed that the tyrosine and cysteine-methionine metabolism pathways were downregulated in pheochromocytoma, whereas the pyrimidine pathway showed no significant difference. Finally, we developed an optimized diagnostic model of two metabolites, L-dihydroorotic acid and vanylglycol. Our results for these metabolites suggest that they may serve as potential clinical biomarkers and can be used to supplement and improve the diagnosis of pheochromocytoma.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Journal of Zhejiang University. Science. B - (2024) vom: 06. März, Seite 1-12 |
| Sprache: |
Englisch |
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| Weiterer Titel: |
肾上腺嗜铬细胞瘤影响三条主要代谢通路：半胱氨酸-蛋氨酸代谢、嘧啶代谢和酪氨酸代谢通路 |
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| Beteiligte Personen: |
Lai, Chong [VerfasserIn] |
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| Links: |
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| Themen: |
Gene set variation analysis |
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| Anmerkungen: |
Date Revised 06.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1631/jzus.B2300579 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Adrenal pheochromocytoma impacts three main pathways |b cysteine-methionine, pyrimidine, and tyrosine metabolism |
| 246 | 3 | 3 | |a 肾上腺嗜铬细胞瘤影响三条主要代谢通路：半胱氨酸-蛋氨酸代谢、嘧啶代谢和酪氨酸代谢通路 |
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| 520 | |a Pheochromocytomas and paragangliomas (PPGLs) cause symptoms by altering the circulation levels of catecholamines and peptide hormones. Currently, the diagnosis of PPGLs relies on diagnostic imaging and the detection of catecholamines. In this study, we used ultra-performance liquid chromatography (UPLC)/quadrupole time-of-flight mass spectrometry (Q-TOF MS) analysis to identify and measure the perioperative differential metabolites in the plasma of adrenal pheochromocytoma patients. We identified differentially expressed genes by comparing the transcriptomic data of pheochromocytoma with the normal adrenal medulla. Through conducting two steps of metabolomics analysis, we identified 111 differential metabolites between the healthy group and the patient group, among which 53 metabolites were validated. By integrating the information of differential metabolites and differentially expressed genes, we inferred that the cysteine-methionine, pyrimidine, and tyrosine metabolism pathways were the three main metabolic pathways altered by the neoplasm. The analysis of transcription levels revealed that the tyrosine and cysteine-methionine metabolism pathways were downregulated in pheochromocytoma, whereas the pyrimidine pathway showed no significant difference. Finally, we developed an optimized diagnostic model of two metabolites, L-dihydroorotic acid and vanylglycol. Our results for these metabolites suggest that they may serve as potential clinical biomarkers and can be used to supplement and improve the diagnosis of pheochromocytoma | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gene set variation analysis | |
| 650 | 4 | |a L-Dihydroorotic acid | |
| 650 | 4 | |a Metabolomics | |
| 650 | 4 | |a Pheochromocytoma and paraganglioma (PPGL) | |
| 650 | 4 | |a Vanylglycol | |
| 700 | 1 | |a Yang, Qingling |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yunuo |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Renjie |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Majie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jiankang |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Maode |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Qingrong |e verfasserin |4 aut | |
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