Development of broad-spectrum immunoassay with monoclonal antibody to detect five eugenols and study of their molecular recognition mechanism
© 2024 The Author(s)..
In this study, three eugenol fragment-containing haptens were synthesized, and a monoclonal antibody (mAb) selective for five commonly-found eugenol compounds (EUGs, i.e., eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, and acetyl isoeugenol) was obtained. Based on this mAb, a broad-spectrum indirect competitive ELISA for high-throughput detection of five EUGs was developed. The detection limits for eugenol, isoeugenol, methyl eugenol, methyl isoeugenol and acetyl isoeugenol in both tilapia and shrimp samples were 25.3/ 50.6 μg/kg, 0.075/0.15 μg/kg, 0.48/0.96 μg/kg, 0.16/0.32 μg/kg, and 18.16/36.32 μg/kg, respectively. The recoveries for five EUGs ranged from 80.4 to 114.0 % with a coefficient of variation less than 11.5 %. Moreover, homology modelling and molecular docking were conducted to elucidate the interactions mechanism of mAb-EUGs. The work provides a promising tool for high-throughput screening of EUGs in aquatic products, which can serve as a benchmark for designing haptens and developing immunoassays for other small molecules.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
Food chemistry: X - 22(2024) vom: 30. März, Seite 101255 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Luo, Lin [VerfasserIn] |
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| Links: |
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| Themen: |
Eugenol |
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| Anmerkungen: |
Date Revised 07.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.fochx.2024.101255 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369343883 |
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| 520 | |a © 2024 The Author(s). | ||
| 520 | |a In this study, three eugenol fragment-containing haptens were synthesized, and a monoclonal antibody (mAb) selective for five commonly-found eugenol compounds (EUGs, i.e., eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, and acetyl isoeugenol) was obtained. Based on this mAb, a broad-spectrum indirect competitive ELISA for high-throughput detection of five EUGs was developed. The detection limits for eugenol, isoeugenol, methyl eugenol, methyl isoeugenol and acetyl isoeugenol in both tilapia and shrimp samples were 25.3/ 50.6 μg/kg, 0.075/0.15 μg/kg, 0.48/0.96 μg/kg, 0.16/0.32 μg/kg, and 18.16/36.32 μg/kg, respectively. The recoveries for five EUGs ranged from 80.4 to 114.0 % with a coefficient of variation less than 11.5 %. Moreover, homology modelling and molecular docking were conducted to elucidate the interactions mechanism of mAb-EUGs. The work provides a promising tool for high-throughput screening of EUGs in aquatic products, which can serve as a benchmark for designing haptens and developing immunoassays for other small molecules | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Eugenol | |
| 650 | 4 | |a Fish anesthetic | |
| 650 | 4 | |a Hapten | |
| 650 | 4 | |a Immunoassay | |
| 650 | 4 | |a Monoclonal antibody | |
| 700 | 1 | |a Kang, Rui-Yao |e verfasserin |4 aut | |
| 700 | 1 | |a He, Zhen-Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Bao-Zhu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zi-Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zhen-Lin |e verfasserin |4 aut | |
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