Identification of biomarkers and pathways for the SARS-CoV-2 infections in obstructive sleep apnea patients based on machine learning and proteomic analysis

© 2024. The Author(s)..

BACKGROUND: The prevalence of obstructive sleep apnea (OSA) was found to be higher in individuals following COVID-19 infection. However, the intricate mechanisms that underscore this concomitance remain partially elucidated. The aim of this study was to delve deeper into the molecular mechanisms that underpin this comorbidity.

METHODS: We acquired gene expression profiles for COVID-19 (GSE157103) and OSA (GSE75097) from the Gene Expression Omnibus (GEO) database. Upon identifying shared feature genes between OSA and COVID-19 utilizing LASSO, Random forest and Support vector machines algorithms, we advanced to functional annotation, analysis of protein-protein interaction networks, module construction, and identification of pivotal genes. Furthermore, we established regulatory networks encompassing transcription factor (TF)-gene and TF-miRNA interactions, and searched for promising drug targets. Subsequently, the expression levels of pivotal genes were validated through proteomics data from COVID-19 cases.

RESULTS: Fourteen feature genes shared between OSA and COVID-19 were selected for further investigation. Through functional annotation, it was indicated that metabolic pathways play a role in the pathogenesis of both disorders. Subsequently, employing the cytoHubba plugin, ten hub genes were recognized, namely TP53, CCND1, MDM2, RB1, HIF1A, EP300, STAT3, CDK2, HSP90AA1, and PPARG. The finding of proteomics unveiled a substantial augmentation in the expression level of HSP90AA1 in COVID-19 patient samples, especially in severe conditions.

CONCLUSIONS: Our investigation illuminate a mutual pathogenic mechanism that underlies both OSA and COVID-19, which may provide novel perspectives for future investigations into the underlying mechanisms.

Medienart:

E-Artikel

Erscheinungsjahr:

2024

Erschienen:

2024

Enthalten in:

Zur Gesamtaufnahme - volume:24

Enthalten in:

BMC pulmonary medicine - 24(2024), 1 vom: 05. März, Seite 112

Sprache:

Englisch

Beteiligte Personen:

Luo, Hong [VerfasserIn]
Yan, Jisong [VerfasserIn]
Gong, Rui [VerfasserIn]
Zhang, Dingyu [VerfasserIn]
Zhou, Xia [VerfasserIn]
Wang, Xianguang [VerfasserIn]

Links:

Volltext

Themen:

Biomarkers
COVID-19
Feature genes
Hub genes
Journal Article
Obstructive sleep apnea
Pathogenesis

Anmerkungen:

Date Completed 07.03.2024

Date Revised 08.03.2024

published: Electronic

Citation Status MEDLINE

doi:

10.1186/s12890-024-02921-1

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM369336836