Effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells
OBJECTIVE: To observe the effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells.
METHODS: One group of L02 cell was treated with different concentrations of selenomethionine(SeMet, 0.001, 0.0025, 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075 and 0.1μmol/L) for 48 h, then cultured with serum-free medium for 4 h and stimulated with 1 μmol/L insulin for 15 min. The insulin signaling pathway(PI3K-AKT-mTOR) was detected by WB. Another group of L02 cell was treated with the same concentrations of SeMet as above for 48 h. The cell supernatant and lysates were collected for the analysis of SELENOP and GPX1, respectively by WB.
RESULTS: The expressions of P-AKT-(Ser-473), P-AKT-(Thr-308), PI3K and mTOR in L02 cells under high-Se were decreased with the increase of SeMet concentration. The expressions of GPX1 and SELENOP were enhanced with the increase of SeMet.
CONCLUSION: The insulin signaling pathway, PI3K-AKT-mTOR, was damaged in L02 cell under high-Se stress.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Wei sheng yan jiu = Journal of hygiene research - 53(2024), 1 vom: 01. Jan., Seite 77-87 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Wang, Qin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.03.2024 Date Revised 07.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.19813/j.cnki.weishengyanjiu.2024.01.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369330129 |
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| 245 | 1 | 0 | |a Effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells |
| 264 | 1 | |c 2024 | |
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| 500 | |a Date Completed 07.03.2024 | ||
| 500 | |a Date Revised 07.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To observe the effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells | ||
| 520 | |a METHODS: One group of L02 cell was treated with different concentrations of selenomethionine(SeMet, 0.001, 0.0025, 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075 and 0.1μmol/L) for 48 h, then cultured with serum-free medium for 4 h and stimulated with 1 μmol/L insulin for 15 min. The insulin signaling pathway(PI3K-AKT-mTOR) was detected by WB. Another group of L02 cell was treated with the same concentrations of SeMet as above for 48 h. The cell supernatant and lysates were collected for the analysis of SELENOP and GPX1, respectively by WB | ||
| 520 | |a RESULTS: The expressions of P-AKT-(Ser-473), P-AKT-(Thr-308), PI3K and mTOR in L02 cells under high-Se were decreased with the increase of SeMet concentration. The expressions of GPX1 and SELENOP were enhanced with the increase of SeMet | ||
| 520 | |a CONCLUSION: The insulin signaling pathway, PI3K-AKT-mTOR, was damaged in L02 cell under high-Se stress | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a L02 cell | |
| 650 | 4 | |a PI3K-AKT-mTOR | |
| 650 | 4 | |a SELENOP | |
| 650 | 4 | |a glutathione peroxidase 1 | |
| 650 | 4 | |a selenium | |
| 650 | 7 | |a Selenium |2 NLM | |
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| 700 | 1 | |a Zhang, Xue |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jianrong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yiqun |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Xiang, Xuesong |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Yanbin |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Zhenwu |e verfasserin |4 aut | |
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