Bulk RNA Sequencing of Human Pediatric Lung Cell Populations Reveals Unique Transcriptomic Signature Associated with Post-natal Pulmonary Development
Post-natal lung development results in an increasingly functional organ prepared for gas exchange and pathogenic challenges. It is achieved through cellular differentiation and migration. Changes in the tissue architecture during this development process are well documented and increasing cellular diversity associated with it are reported in recent years. Despite recent progress, transcriptomic and molecular pathways associated with human post-natal lung development are yet to be fully understood. In this study we investigated gene expression patterns associated with healthy pediatric lung development in four major enriched cell populations (epithelial, endothelial, and non-endothelial mesenchymal cells, along with lung leukocytes) from one-day-old to eight-year-old organ donors with no known lung disease. For analysis, we considered the donors in 4 age groups [less than 30 days old neonates, 30 days to < 1 year old infants, toddlers (1 to < 2 years) and children 2 years and older] and assessed differentially expressed genes (DEG). We found increasing age-associated transcriptional changes in all four major cell types in pediatric lung. Transition from neonate to infant stage showed highest number of DEG compared to number of DEG found during infant to toddler- or toddler to older children- transitions. Profiles of differential gene expression and further pathway enrichment analyses indicate functional epithelial cell maturation and increased capability of antigen presentation and chemokine-mediated communication. Our study provides a comprehensive reference of gene expression patterns during healthy pediatric lung development that will be useful in identifying and understanding aberrant gene expression patterns associated with early life respiratory diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
American journal of physiology. Lung cellular and molecular physiology - (2024) vom: 05. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bandyopadhyay, Gautam [VerfasserIn] |
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| Links: |
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| Themen: |
Human |
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| Anmerkungen: |
Date Revised 05.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1152/ajplung.00385.2023 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369320417 |
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| 245 | 1 | 0 | |a Bulk RNA Sequencing of Human Pediatric Lung Cell Populations Reveals Unique Transcriptomic Signature Associated with Post-natal Pulmonary Development |
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| 520 | |a Post-natal lung development results in an increasingly functional organ prepared for gas exchange and pathogenic challenges. It is achieved through cellular differentiation and migration. Changes in the tissue architecture during this development process are well documented and increasing cellular diversity associated with it are reported in recent years. Despite recent progress, transcriptomic and molecular pathways associated with human post-natal lung development are yet to be fully understood. In this study we investigated gene expression patterns associated with healthy pediatric lung development in four major enriched cell populations (epithelial, endothelial, and non-endothelial mesenchymal cells, along with lung leukocytes) from one-day-old to eight-year-old organ donors with no known lung disease. For analysis, we considered the donors in 4 age groups [less than 30 days old neonates, 30 days to < 1 year old infants, toddlers (1 to < 2 years) and children 2 years and older] and assessed differentially expressed genes (DEG). We found increasing age-associated transcriptional changes in all four major cell types in pediatric lung. Transition from neonate to infant stage showed highest number of DEG compared to number of DEG found during infant to toddler- or toddler to older children- transitions. Profiles of differential gene expression and further pathway enrichment analyses indicate functional epithelial cell maturation and increased capability of antigen presentation and chemokine-mediated communication. Our study provides a comprehensive reference of gene expression patterns during healthy pediatric lung development that will be useful in identifying and understanding aberrant gene expression patterns associated with early life respiratory diseases | ||
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| 650 | 4 | |a Lung development | |
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| 650 | 4 | |a Post-natal lung | |
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| 700 | 1 | |a Jehrio, Matthew G |e verfasserin |4 aut | |
| 700 | 1 | |a Baker, Cameron |e verfasserin |4 aut | |
| 700 | 1 | |a Bhattacharya, Soumyaroop |e verfasserin |4 aut | |
| 700 | 1 | |a Misra, Ravi S |e verfasserin |4 aut | |
| 700 | 1 | |a Huyck, Heidie L |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Chin-Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Myers, Jason R |e verfasserin |4 aut | |
| 700 | 1 | |a Ashton, John |e verfasserin |4 aut | |
| 700 | 1 | |a Polter, Steven |e verfasserin |4 aut | |
| 700 | 1 | |a Cochran, Matthew |e verfasserin |4 aut | |
| 700 | 1 | |a Bushnell, Timothy |e verfasserin |4 aut | |
| 700 | 1 | |a Dutra, Jennifer |e verfasserin |4 aut | |
| 700 | 1 | |a Katzman, Philip J |e verfasserin |4 aut | |
| 700 | 1 | |a Deutsch, Gail H |e verfasserin |4 aut | |
| 700 | 1 | |a Mariani, Thomas J |e verfasserin |4 aut | |
| 700 | 1 | |a Pryhuber, Gloria S |e verfasserin |4 aut | |
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