Ginsenosides Rh1, Rg2, and Rg3 ameliorate dexamethasone-induced muscle atrophy in C2C12 myotubes
© The Korean Society of Food Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law..
High doses or prolonged use of the exogenous synthetic glucocorticoid dexamethasone (Dex) can lead to muscle atrophy. In this study, the anti-atrophic effects of ginsenosides Rh1, Rg2, and Rg3 on Dex-induced C2C12 myotube atrophy were assessed by XTT, myotube diameter, fusion index, and western blot analysis. The XTT assay results showed that treatment with Rh1, Rg2, and Rg3 enhanced cell viability in Dex-injured C2C12 myotubes. Compared with the control group, the myotube diameter and fusion index were both reduced in Dex-treated cells, but treatment with Rh1, Rg2, and Rg3 increased these parameters. Furthermore, Rh1, Rg2, and Rg3 significantly downregulated the protein expression of FoxO3a, MuRF1, and Fbx32, while also upregulating mitochondrial biogenesis through the SIRT1/PGC-1α pathway. It also prevents myotube atrophy by regulating the IGF-1/Akt/ mTOR signaling pathway. These findings indicate that Rh1, Rg2, and Rg3 have great potential as useful agents for the prevention and treatment of muscle atrophy.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
|---|---|
| Enthalten in: |
Food science and biotechnology - 33(2024), 5 vom: 28. März, Seite 1233-1243 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Men, Xiao [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
C2C12 |
|---|
| Anmerkungen: |
Date Revised 06.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1007/s10068-023-01407-w |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM369305442 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM369305442 | ||
| 003 | DE-627 | ||
| 005 | 20240306233339.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240305s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10068-023-01407-w |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1318.xml |
| 035 | |a (DE-627)NLM369305442 | ||
| 035 | |a (NLM)38440685 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Men, Xiao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Ginsenosides Rh1, Rg2, and Rg3 ameliorate dexamethasone-induced muscle atrophy in C2C12 myotubes |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 06.03.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © The Korean Society of Food Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. | ||
| 520 | |a High doses or prolonged use of the exogenous synthetic glucocorticoid dexamethasone (Dex) can lead to muscle atrophy. In this study, the anti-atrophic effects of ginsenosides Rh1, Rg2, and Rg3 on Dex-induced C2C12 myotube atrophy were assessed by XTT, myotube diameter, fusion index, and western blot analysis. The XTT assay results showed that treatment with Rh1, Rg2, and Rg3 enhanced cell viability in Dex-injured C2C12 myotubes. Compared with the control group, the myotube diameter and fusion index were both reduced in Dex-treated cells, but treatment with Rh1, Rg2, and Rg3 increased these parameters. Furthermore, Rh1, Rg2, and Rg3 significantly downregulated the protein expression of FoxO3a, MuRF1, and Fbx32, while also upregulating mitochondrial biogenesis through the SIRT1/PGC-1α pathway. It also prevents myotube atrophy by regulating the IGF-1/Akt/ mTOR signaling pathway. These findings indicate that Rh1, Rg2, and Rg3 have great potential as useful agents for the prevention and treatment of muscle atrophy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a C2C12 | |
| 650 | 4 | |a Dexamethasone | |
| 650 | 4 | |a Ginsenosides Rh1, Rg2, Rg3 | |
| 650 | 4 | |a Muscle atrophy | |
| 650 | 4 | |a Myoblast differentiation | |
| 700 | 1 | |a Han, Xionggao |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Se-Jeong |e verfasserin |4 aut | |
| 700 | 1 | |a Oh, Geon |e verfasserin |4 aut | |
| 700 | 1 | |a Im, Ji-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Bae, Kwi Sik |e verfasserin |4 aut | |
| 700 | 1 | |a Seong, Geum-Su |e verfasserin |4 aut | |
| 700 | 1 | |a La, Im-Joung |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Do-Sang |e verfasserin |4 aut | |
| 700 | 1 | |a Choi, Sun-Il |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Ok-Hwan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food science and biotechnology |d 1998 |g 33(2024), 5 vom: 28. März, Seite 1233-1243 |w (DE-627)NLM097544485 |x 2092-6456 |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2024 |g number:5 |g day:28 |g month:03 |g pages:1233-1243 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10068-023-01407-w |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 33 |j 2024 |e 5 |b 28 |c 03 |h 1233-1243 | ||