Resazurin to determine the minimum inhibitory concentration on antifungal susceptibility assays for Fonsecaea sp. using a modified EUCAST protocol
© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia..
Chromoblastomycosis is a fungal chronic disease, which affects humans, especially in cutaneous and subcutaneous tissues. There is no standard treatment for Chromoblastomycosis, and it is a therapeutic challenge, due natural resistance of their causative agents, inadequate response of patients and common cases of relapse. Protocols for determination of antifungal drugs susceptibility are not standardized for chromoblastomycosis agents and endpoint definition is usually based on visual inspection, which depends on the analyst, making it sometimes inaccurate. We presented a colorimetric and quantitative methodology based on resazurin reduction to resofurin to determine the metabolic status of viable cells of Fonsecaea sp. Performing antifungal susceptibility assay by a modified EUCAST protocol allied to resazurin, we validated the method to identify the minimum inhibitory concentrations of itraconazole, fluconazole, amphotericin B, and terbinafine for eight Fonsecaea clinical isolates. According to our data, resazurin is a good indicator of metabolic status of viable cells, including those exposed to antifungal drugs. This work aimed to test resazurin as an indicator of the metabolic activity of Fonsecaea species in susceptibility assays to antifungal drugs. Species of this genus are the main causative agents of Chromoblastomycosis, which affects humans.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology - (2024) vom: 04. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Herman, Tatiana Sobianski [VerfasserIn] |
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| Links: |
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| Themen: |
Chromoblastomycosis |
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| Anmerkungen: |
Date Revised 04.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1007/s42770-024-01293-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369286944 |
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| 100 | 1 | |a Herman, Tatiana Sobianski |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Resazurin to determine the minimum inhibitory concentration on antifungal susceptibility assays for Fonsecaea sp. using a modified EUCAST protocol |
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| 520 | |a © 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia. | ||
| 520 | |a Chromoblastomycosis is a fungal chronic disease, which affects humans, especially in cutaneous and subcutaneous tissues. There is no standard treatment for Chromoblastomycosis, and it is a therapeutic challenge, due natural resistance of their causative agents, inadequate response of patients and common cases of relapse. Protocols for determination of antifungal drugs susceptibility are not standardized for chromoblastomycosis agents and endpoint definition is usually based on visual inspection, which depends on the analyst, making it sometimes inaccurate. We presented a colorimetric and quantitative methodology based on resazurin reduction to resofurin to determine the metabolic status of viable cells of Fonsecaea sp. Performing antifungal susceptibility assay by a modified EUCAST protocol allied to resazurin, we validated the method to identify the minimum inhibitory concentrations of itraconazole, fluconazole, amphotericin B, and terbinafine for eight Fonsecaea clinical isolates. According to our data, resazurin is a good indicator of metabolic status of viable cells, including those exposed to antifungal drugs. This work aimed to test resazurin as an indicator of the metabolic activity of Fonsecaea species in susceptibility assays to antifungal drugs. Species of this genus are the main causative agents of Chromoblastomycosis, which affects humans | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Fonsecaea sp. | |
| 650 | 4 | |a Chromoblastomycosis | |
| 650 | 4 | |a Metabolic activity | |
| 650 | 4 | |a Resazurin | |
| 650 | 4 | |a Susceptibility to antifungal agents | |
| 700 | 1 | |a da Silva Goersch, Camila |e verfasserin |4 aut | |
| 700 | 1 | |a Bocca, Anamelia Lorenzetti |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Larissa |e verfasserin |4 aut | |
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