A novel ratiometric fluorescent probe with high selectivity for lysosomal nitric oxide imaging
Copyright © 2024. Published by Elsevier B.V..
Nitric oxide (NO) plays critical roles in both physiology and pathology, serving as a significant signaling molecule. Recent investigations have uncovered the pivotal role of lysosome as a critical organelle where intracellular NO exists and takes function. In this study, we developed a novel ratiometric fluorescent probe called XL-NO and modified it with a morpholine unit, which followed the intramolecular charge transfer (ICT) mechanism. The probe could detect lysosomal nitric oxide with high selectivity and sensitivity. The probe XL-NO contained a secondary amine moiety that could readily react with NO in lysosomes, leading to the formation of the N-nitrosation product. The N-nitroso structure enhanced the capability in push-pull electron, which obviously led to the change of fluorescence from 621 nm to 521 nm. In addition, XL-NO was discovered to have some evident advantages, such as significant ratiometric signal (I521/I621) change, strong anti-interference ability, good biocompatibility, and a low detection limit (LOD = 44.3 nM), which were crucial for the detection of lysosomal NO. To evaluate the practical application of XL-NO, NO imaging experiments were performed in both living cells and zebrafish. The results from these experiments confirmed the feasibility and reliability of XL-NO for exogenous/endogenous NO imaging and lysosome targeting.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1297 |
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| Enthalten in: |
Analytica chimica acta - 1297(2024) vom: 08. März, Seite 342303 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xu, Zhiling [VerfasserIn] |
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| Links: |
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| Themen: |
31C4KY9ESH |
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| Anmerkungen: |
Date Completed 06.03.2024 Date Revised 06.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.aca.2024.342303 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Nitric oxide (NO) plays critical roles in both physiology and pathology, serving as a significant signaling molecule. Recent investigations have uncovered the pivotal role of lysosome as a critical organelle where intracellular NO exists and takes function. In this study, we developed a novel ratiometric fluorescent probe called XL-NO and modified it with a morpholine unit, which followed the intramolecular charge transfer (ICT) mechanism. The probe could detect lysosomal nitric oxide with high selectivity and sensitivity. The probe XL-NO contained a secondary amine moiety that could readily react with NO in lysosomes, leading to the formation of the N-nitrosation product. The N-nitroso structure enhanced the capability in push-pull electron, which obviously led to the change of fluorescence from 621 nm to 521 nm. In addition, XL-NO was discovered to have some evident advantages, such as significant ratiometric signal (I521/I621) change, strong anti-interference ability, good biocompatibility, and a low detection limit (LOD = 44.3 nM), which were crucial for the detection of lysosomal NO. To evaluate the practical application of XL-NO, NO imaging experiments were performed in both living cells and zebrafish. The results from these experiments confirmed the feasibility and reliability of XL-NO for exogenous/endogenous NO imaging and lysosome targeting | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biological imaging | |
| 650 | 4 | |a Lysosomes-targeted | |
| 650 | 4 | |a Nitric oxide | |
| 650 | 4 | |a Ratiometric fluorescent probe | |
| 650 | 4 | |a Zebrafish | |
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| 700 | 1 | |a Xu, Liren |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zichun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaoli |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yifan |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Jing, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Guangle |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiaoling |e verfasserin |4 aut | |
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