Photo-sensitive peptide inducing targeted cross-linking in a one-step and reagent-, enzyme- and antibody-free detection of SARS-Cov-2 marker protein
Copyright © 2024 Elsevier B.V. All rights reserved..
Modern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing specific cross-linking upon protease-substrate recognition. This process forms a di-tyrosine product with a distinctive fluorescence signal readout, enabling the detection of SARS-CoV-2 in patient serum samples. This method anticipates a major reduction in assay costs in the near future.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:157 |
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| Enthalten in: |
Bioelectrochemistry (Amsterdam, Netherlands) - 157(2024) vom: 29. März, Seite 108672 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hou, Meihui [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.03.2024 Date Revised 20.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bioelechem.2024.108672 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Modern biosensing technology plays a crucial role in combating the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). However, the associated assays remain costly, considering their extensive daily use. In response, we developed a simplified one-step SARS-CoV-2 protease assay that reduces both time and financial expenses. This approach eliminates the need for extra reagents, enzymes, or antibodies. The simplification involves a photo-sensitive Bengal red-tagged substrate peptide, allowing specific cross-linking upon protease-substrate recognition. This process forms a di-tyrosine product with a distinctive fluorescence signal readout, enabling the detection of SARS-CoV-2 in patient serum samples. This method anticipates a major reduction in assay costs in the near future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a COVID-2019 | |
| 650 | 4 | |a Covalent biosensing | |
| 650 | 4 | |a Peptide | |
| 650 | 4 | |a Photo-induced cross-linking | |
| 650 | 4 | |a Proximity-induce labeling | |
| 650 | 4 | |a SARS-Cov-2 | |
| 650 | 7 | |a Indicators and Reagents |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
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| 700 | 1 | |a Qin, Mingyu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Lei |e verfasserin |4 aut | |
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