The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved..
Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unmapped at a single-cell and spatial resolution. Combining single-cell RNA sequencing, spatial transcriptomics, and multiplexed fluorescent in situ hybridization, we identify three discrete excitatory cell-type clusters that correspond to the known nuclei of the ATN and uncover marker genes, molecular pathways, and putative functions of these cell types. We further illustrate graded spatial variation along the dorsomedial-ventrolateral axis for all individual nuclei of the ATN and additionally demonstrate that the anteroventral nucleus exhibits spatially covarying protein products and long-range inputs. Collectively, our study reveals discrete and continuous cell-type organizational principles of the ATN, which will help to guide and interpret experiments on ATN computation and function.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Cell reports - 43(2024), 3 vom: 26. März, Seite 113842 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kapustina, Margarita [VerfasserIn] |
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| Links: |
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| Themen: |
Anterior thalamic nuclei |
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| Anmerkungen: |
Date Completed 01.04.2024 Date Revised 01.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2024.113842 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369174461 |
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| 245 | 1 | 4 | |a The cell-type-specific spatial organization of the anterior thalamic nuclei of the mouse brain |
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| 500 | |a Date Revised 01.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Understanding the cell-type composition and spatial organization of brain regions is crucial for interpreting brain computation and function. In the thalamus, the anterior thalamic nuclei (ATN) are involved in a wide variety of functions, yet the cell-type composition of the ATN remains unmapped at a single-cell and spatial resolution. Combining single-cell RNA sequencing, spatial transcriptomics, and multiplexed fluorescent in situ hybridization, we identify three discrete excitatory cell-type clusters that correspond to the known nuclei of the ATN and uncover marker genes, molecular pathways, and putative functions of these cell types. We further illustrate graded spatial variation along the dorsomedial-ventrolateral axis for all individual nuclei of the ATN and additionally demonstrate that the anteroventral nucleus exhibits spatially covarying protein products and long-range inputs. Collectively, our study reveals discrete and continuous cell-type organizational principles of the ATN, which will help to guide and interpret experiments on ATN computation and function | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CP: Cell biology | |
| 650 | 4 | |a CP: Neuroscience | |
| 650 | 4 | |a anterior thalamic nuclei | |
| 650 | 4 | |a cell types | |
| 650 | 4 | |a multiplexed fluorescent in situ hybridization | |
| 650 | 4 | |a single-cell RNA sequencing | |
| 650 | 4 | |a spatial transcriptomics | |
| 700 | 1 | |a Zhang, Angela A |e verfasserin |4 aut | |
| 700 | 1 | |a Tsai, Jennifer Y J |e verfasserin |4 aut | |
| 700 | 1 | |a Bristow, Brianna N |e verfasserin |4 aut | |
| 700 | 1 | |a Kraus, Larissa |e verfasserin |4 aut | |
| 700 | 1 | |a Sullivan, Kaitlin E |e verfasserin |4 aut | |
| 700 | 1 | |a Erwin, Sarah R |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lihua |e verfasserin |4 aut | |
| 700 | 1 | |a Stach, Tara R |e verfasserin |4 aut | |
| 700 | 1 | |a Clements, Jody |e verfasserin |4 aut | |
| 700 | 1 | |a Lemire, Andrew L |e verfasserin |4 aut | |
| 700 | 1 | |a Cembrowski, Mark S |e verfasserin |4 aut | |
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