Decade-long WT1-specific CTLs induced by WT1 peptide vaccination
© 2024. Japanese Society of Hematology..
INTRODUCTION: The peptide-based cancer vaccine targeting Wilms' tumor 1 (WT1) is a promising immunotherapeutic strategy for hematological malignancies. It remains unclear how long and to what extent the WT1-specific CD8 + cytotoxic T cell (CTL) persist after WT1 peptide vaccination.
METHODS: The WT1 peptide vaccine was administered with written consent to a patient with CML in the chronic phase who did not respond well to imatinib, and the patient was followed for 12 years after vaccination. Immune monitoring was performed by specific amplification of WT1-specific CTLs using a mixed lymphocyte peptide culture. T-cell receptors (TCRs) of amplified WT1-specific CTLs were analyzed using next-generation sequencing. This study was approved by the Institutional Review Board of our institution.
RESULT: WT1-specific CTLs, which were initially detected during WT1 peptide vaccination, persisted at a frequency of less than 5 cells per 1,000,000 CD8 + T cells for more than 10 years. TCR repertoire analysis confirmed the diversity of WT1-specific CTLs 11 years after vaccination. CTLs exhibited WT1 peptide-specific cytotoxicity in vitro.
CONCLUSION: The WT1 peptide vaccine induced an immune response that persists for more than 10 years, even after cessation of vaccination in the CML patient.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:119 |
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| Enthalten in: |
International journal of hematology - 119(2024), 4 vom: 22. März, Seite 399-406 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Suwabe, Tatsuya [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 25.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s12185-024-03723-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369170970 |
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| 245 | 1 | 0 | |a Decade-long WT1-specific CTLs induced by WT1 peptide vaccination |
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| 520 | |a © 2024. Japanese Society of Hematology. | ||
| 520 | |a INTRODUCTION: The peptide-based cancer vaccine targeting Wilms' tumor 1 (WT1) is a promising immunotherapeutic strategy for hematological malignancies. It remains unclear how long and to what extent the WT1-specific CD8 + cytotoxic T cell (CTL) persist after WT1 peptide vaccination | ||
| 520 | |a METHODS: The WT1 peptide vaccine was administered with written consent to a patient with CML in the chronic phase who did not respond well to imatinib, and the patient was followed for 12 years after vaccination. Immune monitoring was performed by specific amplification of WT1-specific CTLs using a mixed lymphocyte peptide culture. T-cell receptors (TCRs) of amplified WT1-specific CTLs were analyzed using next-generation sequencing. This study was approved by the Institutional Review Board of our institution | ||
| 520 | |a RESULT: WT1-specific CTLs, which were initially detected during WT1 peptide vaccination, persisted at a frequency of less than 5 cells per 1,000,000 CD8 + T cells for more than 10 years. TCR repertoire analysis confirmed the diversity of WT1-specific CTLs 11 years after vaccination. CTLs exhibited WT1 peptide-specific cytotoxicity in vitro | ||
| 520 | |a CONCLUSION: The WT1 peptide vaccine induced an immune response that persists for more than 10 years, even after cessation of vaccination in the CML patient | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Chronic myeloid leukemia | |
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| 700 | 1 | |a Tamura, Suguru |e verfasserin |4 aut | |
| 700 | 1 | |a Katagiri, Takayuki |e verfasserin |4 aut | |
| 700 | 1 | |a Fuse, Kyoko |e verfasserin |4 aut | |
| 700 | 1 | |a Ida-Kurasaki, Tori |e verfasserin |4 aut | |
| 700 | 1 | |a Ushiki, Takashi |e verfasserin |4 aut | |
| 700 | 1 | |a Sone, Hirohito |e verfasserin |4 aut | |
| 700 | 1 | |a Narita, Miwako |e verfasserin |4 aut | |
| 700 | 1 | |a Masuko, Masayoshi |e verfasserin |4 aut | |
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