The positioning mechanics of microtubule asters in Drosophila embryo explants
© 2023, de-Carvalho et al..
Microtubule asters are essential in localizing the action of microtubules in processes including mitosis and organelle positioning. In large cells, such as the one-cell sea urchin embryo, aster dynamics are dominated by hydrodynamic pulling forces. However, in systems with more densely positioned nuclei such as the early Drosophila embryo, which packs around 6000 nuclei within the syncytium in a crystalline-like order, it is unclear what processes dominate aster dynamics. Here, we take advantage of a cell cycle regulation Drosophila mutant to generate embryos with multiple asters, independent from nuclei. We use an ex vivo assay to further simplify this biological system to explore the forces generated by and between asters. Through live imaging, drug and optical perturbations, and theoretical modeling, we demonstrate that these asters likely generate an effective pushing force over short distances.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
eLife - 12(2024) vom: 01. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
de-Carvalho, Jorge [VerfasserIn] |
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| Links: |
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| Themen: |
Active systems |
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| Anmerkungen: |
Date Completed 04.03.2024 Date Revised 17.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.7554/eLife.90541 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369163133 |
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| 245 | 1 | 4 | |a The positioning mechanics of microtubule asters in Drosophila embryo explants |
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| 520 | |a Microtubule asters are essential in localizing the action of microtubules in processes including mitosis and organelle positioning. In large cells, such as the one-cell sea urchin embryo, aster dynamics are dominated by hydrodynamic pulling forces. However, in systems with more densely positioned nuclei such as the early Drosophila embryo, which packs around 6000 nuclei within the syncytium in a crystalline-like order, it is unclear what processes dominate aster dynamics. Here, we take advantage of a cell cycle regulation Drosophila mutant to generate embryos with multiple asters, independent from nuclei. We use an ex vivo assay to further simplify this biological system to explore the forces generated by and between asters. Through live imaging, drug and optical perturbations, and theoretical modeling, we demonstrate that these asters likely generate an effective pushing force over short distances | ||
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| 700 | 1 | |a Saunders, Timothy E |e verfasserin |4 aut | |
| 700 | 1 | |a Telley, Ivo A |e verfasserin |4 aut | |
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