Development of KASP markers assisted with soybean drought tolerance in the germination stage based on GWAS
Copyright © 2024 Jia, Zhou, Xiong, Wang, Xu, Zhang, Liu, Zhang, Wang, Sun and Chen..
Soybean [Glycine max(L.)Merr.] is a leading oil-bearing crop and cultivated globally over a vast scale. The agricultural landscape in China faces a formidable challenge with drought significantly impacting soybean production. In this study, we treated a natural population of 264 Chinese soybean accessions using 15% PEG-6000 and used GR, GE, GI, RGR, RGE, RGI and ASFV as evaluation index. Using the ASFV, we screened 17 strong drought-tolerant soybean germplasm in the germination stage. Leveraging 2,597,425 high-density SNP markers, we conducted Genome-Wide Association Studies (GWAS) and identified 92 SNPs and 9 candidate genes significantly associated with drought tolerance. Furthermore, we developed two KASP markers for S14_5147797 and S18_53902767, which closely linked to drought tolerance. This research not only enriches the pool of soybean germplasm resources but also establishes a robust foundation for the molecular breeding of drought tolerance soybean varieties.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Frontiers in plant science - 15(2024) vom: 26., Seite 1352379 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jia, Qianru [VerfasserIn] |
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| Links: |
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| Themen: |
Drought tolerance |
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| Anmerkungen: |
Date Revised 02.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3389/fpls.2024.1352379 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 Jia, Zhou, Xiong, Wang, Xu, Zhang, Liu, Zhang, Wang, Sun and Chen. | ||
| 520 | |a Soybean [Glycine max(L.)Merr.] is a leading oil-bearing crop and cultivated globally over a vast scale. The agricultural landscape in China faces a formidable challenge with drought significantly impacting soybean production. In this study, we treated a natural population of 264 Chinese soybean accessions using 15% PEG-6000 and used GR, GE, GI, RGR, RGE, RGI and ASFV as evaluation index. Using the ASFV, we screened 17 strong drought-tolerant soybean germplasm in the germination stage. Leveraging 2,597,425 high-density SNP markers, we conducted Genome-Wide Association Studies (GWAS) and identified 92 SNPs and 9 candidate genes significantly associated with drought tolerance. Furthermore, we developed two KASP markers for S14_5147797 and S18_53902767, which closely linked to drought tolerance. This research not only enriches the pool of soybean germplasm resources but also establishes a robust foundation for the molecular breeding of drought tolerance soybean varieties | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a KASP | |
| 650 | 4 | |a drought tolerance | |
| 650 | 4 | |a germination stage | |
| 650 | 4 | |a soybean | |
| 700 | 1 | |a Zhou, Miaomiao |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Yawen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Junyan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Donghe |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hongmei |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qiong |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Huatao |e verfasserin |4 aut | |
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