Early results of the integrative epigenomic-transcriptomic landscape of colorectal adenoma and cancer
©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved..
BACKGROUND: Aberrant methylation is common during the initiation and progression of colorectal cancer (CRC), and detecting these changes that occur during early adenoma (ADE) formation and CRC progression has clinical value.
AIM: To identify potential DNA methylation markers specific to ADE and CRC.
METHODS: Here, we performed SeqCap targeted bisulfite sequencing and RNA-seq analysis of colorectal ADE and CRC samples to profile the epigenomic-transcriptomic landscape.
RESULTS: Comparing 22 CRC and 25 ADE samples, global methylation was higher in the former, but both showed similar methylation patterns regarding differentially methylated gene positions, chromatin signatures, and repeated elements. High-grade CRC tended to exhibit elevated methylation levels in gene promoter regions compared to those in low-grade CRC. Combined with RNA-seq gene expression data, we identified 14 methylation-regulated differentially expressed genes, of which only AGTR1 and NECAB1 methylation had prognostic significance.
CONCLUSION: Our results suggest that genome-wide alterations in DNA methylation occur during the early stages of CRC and demonstrate the methylation signatures associated with colorectal ADEs and CRC, suggesting prognostic biomarkers for CRC.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:16 |
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| Enthalten in: |
World journal of gastrointestinal oncology - 16(2024), 2 vom: 15. Feb., Seite 414-435 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lu, You-Wang [VerfasserIn] |
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| Links: |
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| Themen: |
Colorectal cancer |
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| Anmerkungen: |
Date Revised 08.03.2024 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.4251/wjgo.v16.i2.414 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369152956 |
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| 245 | 1 | 0 | |a Early results of the integrative epigenomic-transcriptomic landscape of colorectal adenoma and cancer |
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| 500 | |a Date Revised 08.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Aberrant methylation is common during the initiation and progression of colorectal cancer (CRC), and detecting these changes that occur during early adenoma (ADE) formation and CRC progression has clinical value | ||
| 520 | |a AIM: To identify potential DNA methylation markers specific to ADE and CRC | ||
| 520 | |a METHODS: Here, we performed SeqCap targeted bisulfite sequencing and RNA-seq analysis of colorectal ADE and CRC samples to profile the epigenomic-transcriptomic landscape | ||
| 520 | |a RESULTS: Comparing 22 CRC and 25 ADE samples, global methylation was higher in the former, but both showed similar methylation patterns regarding differentially methylated gene positions, chromatin signatures, and repeated elements. High-grade CRC tended to exhibit elevated methylation levels in gene promoter regions compared to those in low-grade CRC. Combined with RNA-seq gene expression data, we identified 14 methylation-regulated differentially expressed genes, of which only AGTR1 and NECAB1 methylation had prognostic significance | ||
| 520 | |a CONCLUSION: Our results suggest that genome-wide alterations in DNA methylation occur during the early stages of CRC and demonstrate the methylation signatures associated with colorectal ADEs and CRC, suggesting prognostic biomarkers for CRC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Colorectal cancer | |
| 650 | 4 | |a Epigenomic alteration | |
| 650 | 4 | |a Methylation-regulated differentially expressed genes | |
| 650 | 4 | |a Transcriptome | |
| 700 | 1 | |a Ding, Zhao-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Gui-Gang |e verfasserin |4 aut | |
| 700 | 1 | |a He, Yu-Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiao-Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jiang |e verfasserin |4 aut | |
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