IFN-γ decreases PD-1 in T lymphocytes from convalescent COVID-19 patients via the AKT/GSK3β signaling pathway
© 2024. The Author(s)..
Post-COVID-19 syndrome may be associated with the abnormal immune status. Compared with the unexposed age-matched elder group, PD-1 in the CD8+ T cells from recovered COVID-19 patients was significantly lower. IFN-γ in the plasma of COVID-19 convalescent patients was increased, which inhibited PD-1 expression in CD8+ T cells from COVID-19 convalescent patients. scRNA-seq bioinformatics analysis revealed that AKT/GSK3β may regulate the INF-γ/PD-1 axis in CD8+ T cells from COVID-19 convalescent patients. In parallel, an IFN-γ neutralizing antibody reduced AKT and increased GSK3β in PBMCs. An AKT agonist (SC79) significantly decreased p-GSK3β. Moreover, AKT decreased PD-1 on CD8+ T cells, and GSK3β increased PD-1 on CD8+ T cells according to flow cytometry analysis. Collectively, we demonstrated that recovered COVID-19 patients may develop long COVID. Increased IFN-γ in the plasma of recovered Wuhan COVID-19 patients contributed to PD-1 downregulation on CD8+ T cells by regulating the AKT/GSK3β signaling pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Scientific reports - 14(2024), 1 vom: 29. Feb., Seite 5038 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Song, Meijuan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.03.2024 Date Revised 05.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-024-55191-6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM369139984 |
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| 520 | |a Post-COVID-19 syndrome may be associated with the abnormal immune status. Compared with the unexposed age-matched elder group, PD-1 in the CD8+ T cells from recovered COVID-19 patients was significantly lower. IFN-γ in the plasma of COVID-19 convalescent patients was increased, which inhibited PD-1 expression in CD8+ T cells from COVID-19 convalescent patients. scRNA-seq bioinformatics analysis revealed that AKT/GSK3β may regulate the INF-γ/PD-1 axis in CD8+ T cells from COVID-19 convalescent patients. In parallel, an IFN-γ neutralizing antibody reduced AKT and increased GSK3β in PBMCs. An AKT agonist (SC79) significantly decreased p-GSK3β. Moreover, AKT decreased PD-1 on CD8+ T cells, and GSK3β increased PD-1 on CD8+ T cells according to flow cytometry analysis. Collectively, we demonstrated that recovered COVID-19 patients may develop long COVID. Increased IFN-γ in the plasma of recovered Wuhan COVID-19 patients contributed to PD-1 downregulation on CD8+ T cells by regulating the AKT/GSK3β signaling pathway | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Shen, Weiyu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhengxia |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Jingxian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yanan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Tao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Mingshun |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Mao |e verfasserin |4 aut | |
| 700 | 1 | |a Ji, Ningfei |e verfasserin |4 aut | |
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