Transcriptomic Insights into Metabolism-Dependent Biosynthesis of Bacterial Nanocellulose
Bacterial nanocellulose (BNC) is an attractive green-synthesized biomaterial for biomedical applications and various other applications. However, effective engineering of BNC production has been limited by our poor knowledge of the related metabolic processes. In contrast to the traditional perception that genome critically determines biosynthesis behaviors, here we discover that the glucose metabolism could also drastically affect the BNC synthesis in Gluconacetobacter hansenii. The transcriptomic profiles of two model BNC-producing strains, G. hansenii ATCC 53582 and ATCC 23769, which have highly similar genomes but drastically different BNC yields, were compared. The results show that their BNC synthesis capacities were highly related to metabolic activities such as ATP synthesis, ion transport protein assembly, and carbohydrate metabolic processes, confirming an important role of metabolism-related transcriptomes in governing the BNC yield. Our findings provide insights into the microbial biosynthesis behaviors from a transcriptome perspective, potentially guiding cellular engineering for biomaterial synthesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
ACS applied bio materials - 7(2024), 3 vom: 18. März, Seite 1801-1809 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Qi-Zhong [VerfasserIn] |
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| Links: |
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| Themen: |
BNC-related genes |
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| Anmerkungen: |
Date Completed 19.03.2024 Date Revised 19.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acsabm.3c01222 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM369067010 |
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| 520 | |a Bacterial nanocellulose (BNC) is an attractive green-synthesized biomaterial for biomedical applications and various other applications. However, effective engineering of BNC production has been limited by our poor knowledge of the related metabolic processes. In contrast to the traditional perception that genome critically determines biosynthesis behaviors, here we discover that the glucose metabolism could also drastically affect the BNC synthesis in Gluconacetobacter hansenii. The transcriptomic profiles of two model BNC-producing strains, G. hansenii ATCC 53582 and ATCC 23769, which have highly similar genomes but drastically different BNC yields, were compared. The results show that their BNC synthesis capacities were highly related to metabolic activities such as ATP synthesis, ion transport protein assembly, and carbohydrate metabolic processes, confirming an important role of metabolism-related transcriptomes in governing the BNC yield. Our findings provide insights into the microbial biosynthesis behaviors from a transcriptome perspective, potentially guiding cellular engineering for biomaterial synthesis | ||
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| 700 | 1 | |a Cao, Li-Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hui-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Wen-Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Sheng, Guo-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yin-Guang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wen-Wei |e verfasserin |4 aut | |
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