Discovery of a Pimaradiene that Decreases Viability of MDA-MB-468 Cells Through Inhibition of EGFR Signaling Pathway
© 2024 Wiley‐VHCA AG, Zurich, Switzerland..
Triple-negative breast cancer (TNBC) is characterized by strong invasiveness, high relapse rates, and poor overall survival. It occurs in approximately 15-20 % of all breast cancer cases. Natural compounds are a promising option for managing breast cancer. ent-8(14),15-Pimaradiene-2β,19-diol (JXE-23), is a pimaradiene isolated from the fern Aleuritopteris albofusca. However, the effects and molecular mechanisms of JXE-23 on cancer cells are still unknown. Thus, this study was designed to determine the potential of JXE-23 for its anticancer properties in TNBC cells. JXE-23 was evaluated for its antiproliferative activity in vitro against human breast cancer cell lines, and showed selectively cytotoxic activity against MDA-MB-468, an EGFR-overexpressing TNBC cancer cell line, with an IC50 value of 1.17±0.04 μM. Moreover, mechanistic investigations indicated that JXE-23 was significantly capable of inhibiting cell proliferation and viability in MDA-MB-468 cells. In addition, JXE-23 exerted an anticancer effect against MDA-MB-468 cells via restraining cell migration in a dose-dependent mode. Moreover, after treatment with JXE-23, the protein expressions of pEGFR, pERK, pAkt and p-p70S6K were significantly reduced in MDA-MB-468 cells. The results underscored that JXE-23 could be a potential lead compound for the treatment of EGFR-overexpressing TNBC cells.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
---|---|
Enthalten in: |
Chemistry & biodiversity - 21(2024), 4 vom: 24. Apr., Seite e202400288 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Wang, Nina [VerfasserIn] |
---|
Links: |
---|
Themen: |
Breast cancer |
---|
Anmerkungen: |
Date Completed 18.04.2024 Date Revised 18.04.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1002/cbdv.202400288 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM369058690 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM369058690 | ||
003 | DE-627 | ||
005 | 20240418232641.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240229s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1002/cbdv.202400288 |2 doi | |
028 | 5 | 2 | |a pubmed24n1379.xml |
035 | |a (DE-627)NLM369058690 | ||
035 | |a (NLM)38415947 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Wang, Nina |e verfasserin |4 aut | |
245 | 1 | 0 | |a Discovery of a Pimaradiene that Decreases Viability of MDA-MB-468 Cells Through Inhibition of EGFR Signaling Pathway |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 18.04.2024 | ||
500 | |a Date Revised 18.04.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2024 Wiley‐VHCA AG, Zurich, Switzerland. | ||
520 | |a Triple-negative breast cancer (TNBC) is characterized by strong invasiveness, high relapse rates, and poor overall survival. It occurs in approximately 15-20 % of all breast cancer cases. Natural compounds are a promising option for managing breast cancer. ent-8(14),15-Pimaradiene-2β,19-diol (JXE-23), is a pimaradiene isolated from the fern Aleuritopteris albofusca. However, the effects and molecular mechanisms of JXE-23 on cancer cells are still unknown. Thus, this study was designed to determine the potential of JXE-23 for its anticancer properties in TNBC cells. JXE-23 was evaluated for its antiproliferative activity in vitro against human breast cancer cell lines, and showed selectively cytotoxic activity against MDA-MB-468, an EGFR-overexpressing TNBC cancer cell line, with an IC50 value of 1.17±0.04 μM. Moreover, mechanistic investigations indicated that JXE-23 was significantly capable of inhibiting cell proliferation and viability in MDA-MB-468 cells. In addition, JXE-23 exerted an anticancer effect against MDA-MB-468 cells via restraining cell migration in a dose-dependent mode. Moreover, after treatment with JXE-23, the protein expressions of pEGFR, pERK, pAkt and p-p70S6K were significantly reduced in MDA-MB-468 cells. The results underscored that JXE-23 could be a potential lead compound for the treatment of EGFR-overexpressing TNBC cells | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a EGFR | |
650 | 4 | |a breast cancer | |
650 | 4 | |a cell proliferation | |
650 | 4 | |a natural product | |
650 | 4 | |a pimaradiene | |
650 | 7 | |a ErbB Receptors |2 NLM | |
650 | 7 | |a EC 2.7.10.1 |2 NLM | |
650 | 7 | |a EGFR protein, human |2 NLM | |
650 | 7 | |a EC 2.7.10.1 |2 NLM | |
700 | 1 | |a Qin, Li |e verfasserin |4 aut | |
700 | 1 | |a Liu, Zi |e verfasserin |4 aut | |
700 | 1 | |a Cao, Jianguo |e verfasserin |4 aut | |
700 | 1 | |a Huang, Jiayi |e verfasserin |4 aut | |
700 | 1 | |a Ma, Liang |e verfasserin |4 aut | |
700 | 1 | |a Huang, Guozheng |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Chemistry & biodiversity |d 2004 |g 21(2024), 4 vom: 24. Apr., Seite e202400288 |w (DE-627)NLM167405101 |x 1612-1880 |7 nnns |
773 | 1 | 8 | |g volume:21 |g year:2024 |g number:4 |g day:24 |g month:04 |g pages:e202400288 |
856 | 4 | 0 | |u http://dx.doi.org/10.1002/cbdv.202400288 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 21 |j 2024 |e 4 |b 24 |c 04 |h e202400288 |