Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net..
BACKGROUND: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite.
OBJECTIVE: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis.
METHODS: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH.
RESULTS: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs' clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity.
CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Current pharmaceutical biotechnology - (2024) vom: 23. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Moura, Henrique Bonifácio [VerfasserIn] |
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| Links: |
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| Themen: |
Canine visceral leishmaniasis |
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| Anmerkungen: |
Date Revised 27.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.2174/0113892010268142231226115140 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368996832 |
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| 100 | 1 | |a Moura, Henrique Bonifácio |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Diagnosis of Canine Visceral Leishmaniasis by Flow Cytometry Serology using the rMELEISH Multiepitope Antigen Coupled in a Functional Bead |
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| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net. | ||
| 520 | |a BACKGROUND: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite | ||
| 520 | |a OBJECTIVE: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis | ||
| 520 | |a METHODS: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH | ||
| 520 | |a RESULTS: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs' clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity | ||
| 520 | |a CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Leishmania infantum | |
| 650 | 4 | |a canine visceral leishmaniasis | |
| 650 | 4 | |a diagnosis | |
| 650 | 4 | |a dogs | |
| 650 | 4 | |a flow cytometry serology | |
| 650 | 4 | |a zoonotic disease. | |
| 700 | 1 | |a Ker, Henrique Gama |e verfasserin |4 aut | |
| 700 | 1 | |a Ramos, Luana S |e verfasserin |4 aut | |
| 700 | 1 | |a Nogueira, Lais M |e verfasserin |4 aut | |
| 700 | 1 | |a Machado, Juliana M |e verfasserin |4 aut | |
| 700 | 1 | |a Dias, Daniel S |e verfasserin |4 aut | |
| 700 | 1 | |a Rodrigues, Rita Alaíde Leandro |e verfasserin |4 aut | |
| 700 | 1 | |a Paz, Mariana Campos da |e verfasserin |4 aut | |
| 700 | 1 | |a Teixeira-Neto, Rafael Gonçalves |e verfasserin |4 aut | |
| 700 | 1 | |a Reis, Alexandre Barbosa |e verfasserin |4 aut | |
| 700 | 1 | |a Galdino, Alexsandro S |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Eduardo S |e verfasserin |4 aut | |
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