Bright and stable monomeric green fluorescent protein derived from StayGold
© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc..
The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditis elegans and mice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
Nature methods - 21(2024), 4 vom: 26. Apr., Seite 657-665 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Hanbin [VerfasserIn] |
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| Links: |
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| Themen: |
147336-22-9 |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41592-024-02203-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM368991865 |
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| 520 | |a The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditis elegans and mice | ||
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| 700 | 1 | |a Lesnov, Gleb D |e verfasserin |4 aut | |
| 700 | 1 | |a Subach, Oksana M |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenhao |e verfasserin |4 aut | |
| 700 | 1 | |a Kuzmicheva, Tatyana P |e verfasserin |4 aut | |
| 700 | 1 | |a Vlaskina, Anna V |e verfasserin |4 aut | |
| 700 | 1 | |a Samygina, Valeriya R |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Liangyi |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Xianxin |e verfasserin |4 aut | |
| 700 | 1 | |a Nikolaeva, Alena Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Gabdulkhakov, Azat |e verfasserin |4 aut | |
| 700 | 1 | |a Papadaki, Stavrini |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Wenming |e verfasserin |4 aut | |
| 700 | 1 | |a Borshchevskiy, Valentin |e verfasserin |4 aut | |
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| 700 | 1 | |a Drobizhev, Mikhail |e verfasserin |4 aut | |
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| 700 | 1 | |a Piatkevich, Kiryl D |e verfasserin |4 aut | |
| 700 | 1 | |a Subach, Fedor V |e verfasserin |4 aut | |
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