Estrogen receptor GPR30 in the anterior cingulate cortex mediates exacerbated neuropathic pain in ovariectomized mice
Copyright © 2024 Elsevier B.V. All rights reserved..
Menopausal women experience neuropathic pain 63% more frequently than men do, which may attribute to the estrogen withdrawal. However, the underlying mechanisms remain unclear. Here, the role of estrogen receptors (ERs) in ovariectomized (OVX) female mice following chronic constriction injury (CCI) was investigated. With 17β-estradiol (E2) supplemented, aggravated mechanical allodynia in OVX mice could be significantly alleviated, particularly after intra-anterior cingulate cortex (ACC) E2 delivery. Pharmacological interventions further demonstrated that the agonist of G-protein-coupled estrogen receptor 30 (GPR30), rather than ERα or ERβ in the ACC, exhibited the similar analgesic effect as E2, whereas antagonist of GPR30 exacerbated allodynia. Furthermore, OVX surgery reduced GPR30 expression in the ACC, which could be restored with estrogen supplementation. Selective downregulation of GPR30 in the ACC of naïve female mice induces mechanical allodynia, whereas GPR30 overexpression in the ACC remarkedly alleviated OVX-exacerbated allodynia. Collectively, estrogen withdrawal could downregulate the ACC GPR30 expression, resulting in exacerbated neuropathic pain. Our findings highlight the importance of GPR30 in the ACC in aggravated neuropathic pain during menopause, and offer a potential therapeutic candidate for neuropathic pain management in menopausal women.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1829 |
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| Enthalten in: |
Brain research - 1829(2024) vom: 15. März, Seite 148798 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Jiajia [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 26.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.brainres.2024.148798 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Menopausal women experience neuropathic pain 63% more frequently than men do, which may attribute to the estrogen withdrawal. However, the underlying mechanisms remain unclear. Here, the role of estrogen receptors (ERs) in ovariectomized (OVX) female mice following chronic constriction injury (CCI) was investigated. With 17β-estradiol (E2) supplemented, aggravated mechanical allodynia in OVX mice could be significantly alleviated, particularly after intra-anterior cingulate cortex (ACC) E2 delivery. Pharmacological interventions further demonstrated that the agonist of G-protein-coupled estrogen receptor 30 (GPR30), rather than ERα or ERβ in the ACC, exhibited the similar analgesic effect as E2, whereas antagonist of GPR30 exacerbated allodynia. Furthermore, OVX surgery reduced GPR30 expression in the ACC, which could be restored with estrogen supplementation. Selective downregulation of GPR30 in the ACC of naïve female mice induces mechanical allodynia, whereas GPR30 overexpression in the ACC remarkedly alleviated OVX-exacerbated allodynia. Collectively, estrogen withdrawal could downregulate the ACC GPR30 expression, resulting in exacerbated neuropathic pain. Our findings highlight the importance of GPR30 in the ACC in aggravated neuropathic pain during menopause, and offer a potential therapeutic candidate for neuropathic pain management in menopausal women | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anterior cingulate cortex | |
| 650 | 4 | |a Estrogen | |
| 650 | 4 | |a G protein-coupled receptor 30 | |
| 650 | 4 | |a Neuropathic pain | |
| 650 | 4 | |a Ovariectomized mice | |
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| 700 | 1 | |a Wang, Shiquan |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Zongping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jianshuai |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Yaru |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qun |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Zhenhua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Haixing |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Wugang |e verfasserin |4 aut | |
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