Sequence introgression from exogenous lineages underlies genomic and biological differences among Cryptosporidium parvum IOWA lines
Copyright © 2024 Elsevier Ltd. All rights reserved..
The IOWA strain of Cryptosporidium parvum is widely used in studies of the biology and detection of the waterborne pathogens Cryptosporidium spp. While several lines of the strain have been sequenced, IOWA-II, the only reference of the original subtype (IIaA15G2R1), exhibits significant assembly errors. Here we generated a fully assembled genome of IOWA-CDC of this subtype using PacBio and Illumina technologies. In comparative analyses of seven IOWA lines maintained in different laboratories (including two sequenced in this study) and 56 field isolates, IOWA lines (IIaA17G2R1) with less virulence had mixed genomes closely related to IOWA-CDC but with multiple sequence introgressions from IOWA-II and unknown lineages. In addition, the IOWA-IIaA17G2R1 lines showed unique nucleotide substitutions and loss of a gene associated with host infectivity, which were not observed in other isolates analyzed. These genomic differences among IOWA lines could be the genetic determinants of phenotypic traits in C. parvum. These data provide a new reference for comparative genomic analyses of Cryptosporidium spp. and rich targets for the development of advanced source tracking tools.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:254 |
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| Enthalten in: |
Water research - 254(2024) vom: 01. Mai, Seite 121333 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Wanyi [VerfasserIn] |
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| Links: |
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| Themen: |
Cryptosporidium parvum |
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| Anmerkungen: |
Date Completed 08.04.2024 Date Revised 02.05.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.watres.2024.121333 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM368927326 |
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| 520 | |a The IOWA strain of Cryptosporidium parvum is widely used in studies of the biology and detection of the waterborne pathogens Cryptosporidium spp. While several lines of the strain have been sequenced, IOWA-II, the only reference of the original subtype (IIaA15G2R1), exhibits significant assembly errors. Here we generated a fully assembled genome of IOWA-CDC of this subtype using PacBio and Illumina technologies. In comparative analyses of seven IOWA lines maintained in different laboratories (including two sequenced in this study) and 56 field isolates, IOWA lines (IIaA17G2R1) with less virulence had mixed genomes closely related to IOWA-CDC but with multiple sequence introgressions from IOWA-II and unknown lineages. In addition, the IOWA-IIaA17G2R1 lines showed unique nucleotide substitutions and loss of a gene associated with host infectivity, which were not observed in other isolates analyzed. These genomic differences among IOWA lines could be the genetic determinants of phenotypic traits in C. parvum. These data provide a new reference for comparative genomic analyses of Cryptosporidium spp. and rich targets for the development of advanced source tracking tools | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Evolution | |
| 650 | 4 | |a Genome | |
| 650 | 4 | |a IOWA strain | |
| 650 | 4 | |a Virulence determinants | |
| 700 | 1 | |a Tang, Kevin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chengyi |e verfasserin |4 aut | |
| 700 | 1 | |a Arrowood, Michael J |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Yaqiong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Roellig, Dawn M |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Yaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Lihua |e verfasserin |4 aut | |
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