ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved..
Intricate cerebral cortex formation is orchestrated by the precise behavior and division dynamics of radial glial cells (RGCs). Endocytosis functions in the recycling and remodeling of adherens junctions (AJs) in response to changes in RGC activity and function. Here, we show that conditional disruption of ubiquitin-associated protein 1 (UBAP1), a component of endosomal sorting complex required for transport (ESCRT), causes severe brain dysplasia and prenatal ventriculomegaly. UBAP1 depletion disrupts the AJs and polarity of RGCs, leading to failure of apically directed interkinetic nuclear migration. Accordingly, UBAP1 knockout or knockdown results in reduced proliferation and precocious differentiation of neural progenitor cells. Mechanistically, UBAP1 regulates the expression and surface localization of cell adhesion molecules, and β-catenin over-expression significantly rescues the phenotypes of Ubap1 knockdown in vivo. Our study reveals a critical physiological role of the ESCRT machinery in cortical neurogenesis by regulating AJs of RGCs.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Cell reports - 43(2024), 3 vom: 26. März, Seite 113818 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lu, Danping [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.04.2024 Date Revised 01.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2024.113818 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368925617 |
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| 100 | 1 | |a Lu, Danping |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Intricate cerebral cortex formation is orchestrated by the precise behavior and division dynamics of radial glial cells (RGCs). Endocytosis functions in the recycling and remodeling of adherens junctions (AJs) in response to changes in RGC activity and function. Here, we show that conditional disruption of ubiquitin-associated protein 1 (UBAP1), a component of endosomal sorting complex required for transport (ESCRT), causes severe brain dysplasia and prenatal ventriculomegaly. UBAP1 depletion disrupts the AJs and polarity of RGCs, leading to failure of apically directed interkinetic nuclear migration. Accordingly, UBAP1 knockout or knockdown results in reduced proliferation and precocious differentiation of neural progenitor cells. Mechanistically, UBAP1 regulates the expression and surface localization of cell adhesion molecules, and β-catenin over-expression significantly rescues the phenotypes of Ubap1 knockdown in vivo. Our study reveals a critical physiological role of the ESCRT machinery in cortical neurogenesis by regulating AJs of RGCs | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CP: Developmental biology | |
| 650 | 4 | |a CP: Neuroscience | |
| 650 | 4 | |a ESCRT | |
| 650 | 4 | |a UBAP1 | |
| 650 | 4 | |a adherens junctions | |
| 650 | 4 | |a cortical neurogenesis | |
| 650 | 4 | |a interkinetic nuclear migration | |
| 650 | 4 | |a prenatal ventriculomegaly | |
| 650 | 4 | |a radial glial cell | |
| 650 | 4 | |a β-catenin | |
| 650 | 7 | |a Endosomal Sorting Complexes Required for Transport |2 NLM | |
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| 650 | 7 | |a UBAP1 protein, human |2 NLM | |
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| 700 | 1 | |a Zhi, Yiqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Huizhen |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Wenxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Chaoyin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tongtong |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Zhifei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Li-Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Zhiqi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zhiheng |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wanjin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Dan |e verfasserin |4 aut | |
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