Application of confocal microscopy and flow cytometry to identify physiological responses of Prorocentrum micans to the herbicide glyphosate
Copyright © 2024 Elsevier Ltd. All rights reserved..
The physiological response of the dinoflagellate P. micans to the effect of the herbicide glyphosate at a concentration of 25-200 μg L-1 was evaluated. It has been shown that P. micans is able to grow due to the consumption of dissolved organic phosphorus formed as a result of the mineralization of glyphosate by bacteria. The addition of glyphosate to the medium inhibits the photosynthetic activity of cells; there is a pronounced inhibition of the relative electron transfer rate along the electron transport chain and the maximum quantum efficiency of the use of light energy. Morphological and ultrastructural changes in P. micans cells were evaluated at sublethal (150 μg L-1) and lethal (200 μg L-1) glyphosate concentrations. It has been shown that at a herbicide concentration of 150 μg L-1, the first signs of apoptosis appear in most P. micans cells: a decrease in lateral light scattering, cytoplasmic retraction, partial destruction of cytoplasmic organelles, a change in the morphology of nuclei, mitochondria, a change in the potential of mitochondrial membranes, and a decrease in the autofluorescence of chlorophyll in cells. At a glyphosate concentration of 200 μg L-1, P. micans showed signs of a late stage of apoptosis: violation of the integrity of intracellular organelles and chromatin organization, fragmentation of nuclei, condensation of cytoplasm, disorganization of chloroplasts in the cells, and the release of cell contents beyond the cell membrane. The effectiveness of using flow cytometry and laser scanning confocal microscopy methods for identifying signs and stages of cell apoptosis when exposed to glyphosate is discussed.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:196 |
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| Enthalten in: |
Marine environmental research - 196(2024) vom: 30. März, Seite 106417 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Solomonova, Ekaterina [VerfasserIn] |
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| Links: |
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| Themen: |
4632WW1X5A |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 25.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.marenvres.2024.106417 |
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| funding: |
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| 520 | |a The physiological response of the dinoflagellate P. micans to the effect of the herbicide glyphosate at a concentration of 25-200 μg L-1 was evaluated. It has been shown that P. micans is able to grow due to the consumption of dissolved organic phosphorus formed as a result of the mineralization of glyphosate by bacteria. The addition of glyphosate to the medium inhibits the photosynthetic activity of cells; there is a pronounced inhibition of the relative electron transfer rate along the electron transport chain and the maximum quantum efficiency of the use of light energy. Morphological and ultrastructural changes in P. micans cells were evaluated at sublethal (150 μg L-1) and lethal (200 μg L-1) glyphosate concentrations. It has been shown that at a herbicide concentration of 150 μg L-1, the first signs of apoptosis appear in most P. micans cells: a decrease in lateral light scattering, cytoplasmic retraction, partial destruction of cytoplasmic organelles, a change in the morphology of nuclei, mitochondria, a change in the potential of mitochondrial membranes, and a decrease in the autofluorescence of chlorophyll in cells. At a glyphosate concentration of 200 μg L-1, P. micans showed signs of a late stage of apoptosis: violation of the integrity of intracellular organelles and chromatin organization, fragmentation of nuclei, condensation of cytoplasm, disorganization of chloroplasts in the cells, and the release of cell contents beyond the cell membrane. The effectiveness of using flow cytometry and laser scanning confocal microscopy methods for identifying signs and stages of cell apoptosis when exposed to glyphosate is discussed | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a Bacteria | |
| 650 | 4 | |a Flow cytometry | |
| 650 | 4 | |a Glyphosate | |
| 650 | 4 | |a Laser confocal scanning microscopy | |
| 650 | 4 | |a Microalgae | |
| 650 | 4 | |a Vital dyes | |
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| 650 | 7 | |a Herbicides |2 NLM | |
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| 700 | 1 | |a Akimov, Arkady |e verfasserin |4 aut | |
| 700 | 1 | |a Rylkova, Olga |e verfasserin |4 aut | |
| 700 | 1 | |a Meger, Yakov |e verfasserin |4 aut | |
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