An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved..
Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions from cells, extracting DNA using columns, and quantifying extracted DNA using qPCR. This straightforward protocol can be completed in as little as 5 hours, and allows for the identification of the source of DNA. For complete details on the use and execution of this protocol, please refer to Jahun et al.1.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:5 |
|---|---|
| Enthalten in: |
STAR protocols - 5(2024), 1 vom: 15. März, Seite 102913 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Jahun, Aminu S [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
9007-49-2 |
|---|
| Anmerkungen: |
Date Completed 20.03.2024 Date Revised 20.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.xpro.2024.102913 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM368839370 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM368839370 | ||
| 003 | DE-627 | ||
| 005 | 20240320234336.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240229s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.xpro.2024.102913 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1337.xml |
| 035 | |a (DE-627)NLM368839370 | ||
| 035 | |a (NLM)38393950 | ||
| 035 | |a (PII)S2666-1667(24)00078-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Jahun, Aminu S |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An optimized protocol for the extraction and quantification of cytosolic DNA in mammalian cells |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.03.2024 | ||
| 500 | |a Date Revised 20.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Leakage of mitochondrial or nuclear DNA into the cytosol can occur following viral infections, radiation damage, and some cancers. Here, we present an optimized protocol for isolating and quantifying cytosolic DNA from mammalian cells. We describe steps for collecting cytosolic fractions from cells, extracting DNA using columns, and quantifying extracted DNA using qPCR. This straightforward protocol can be completed in as little as 5 hours, and allows for the identification of the source of DNA. For complete details on the use and execution of this protocol, please refer to Jahun et al.1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cancer | |
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a Cell separation/fractionation | |
| 650 | 4 | |a Cell-based Assays | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Molecular Biology | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 700 | 1 | |a Sorgeloos, Frederic |e verfasserin |4 aut | |
| 700 | 1 | |a Goodfellow, Ian G |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t STAR protocols |d 2020 |g 5(2024), 1 vom: 15. März, Seite 102913 |w (DE-627)NLM31262266X |x 2666-1667 |7 nnns |
| 773 | 1 | 8 | |g volume:5 |g year:2024 |g number:1 |g day:15 |g month:03 |g pages:102913 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.xpro.2024.102913 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 5 |j 2024 |e 1 |b 15 |c 03 |h 102913 | ||