Interleukin-19 enhances eosinophil infiltration through upregulation of epithelium-derived RANTES expression via the ERK/NF-κB signalling pathway in patients with eosinophilic CRSwNP
© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG..
BACKGROUD: The recurrence rate of chronic rhinosinusitis with nasal polyps (CRSwNP) is positively correlated with eosinophil infiltration. Increased interleukin (IL)-19 and eosinophil chemokine RANTES levels have been reported in patients with CRSwNP. This study aimed to clarify the role of IL-19 in mediating RANTES expression and eosinophilic infiltration in eosinophilic CRSwNP (Eos CRSwNP).
METHODS: Nasal tissue samples were obtained from patients with CRSwNP and controls. The expression of IL-19, its receptors, ECP, and RANTES in tissues was investigated. Primary human nasal epithelial cells (HNECs) and nasal polyp tissue blocks were cultured, then stimulated by IL-19; ERK phosphorylation, NF-κB pathway activation, RANTES level, eosinophils migration and infiltration were detected using RT-qPCR, ELISA, western blotting, HE, immunohistochemistry, immunofluorescence staining, confocal microscopy, and transwell migration assay.
RESULTS: The expression of IL-19 and its receptors (IL-20R1/IL-20R2), eosinophil cationic protein, and RANTES in nasal tissues from patients with Eos CRSwNP was significantly increased compared to that in non-Eos CRSwNP and control subjects. IL-19 co-localized with RANTES in nasal tissues and significantly elevated RANTES expression in HNECs. IL-19-blocking antibody and siRNA knockdown of IL-20R1 ameliorated the effect of IL-19 on RANTES secretion in HNECs. Moreover, IL-19-induced RANTES upregulation was associated with the activation of the ERK and NF-κB pathways. NF-κB activation was mediated by the ERK pathway in IL-19-treated HNECs, and IL-19 enhanced eosinophil infiltration in nasal polyp tissue blocks.
CONCLUSIONS: Our findings indicate that IL-19 promotes RANTES expression via the ERK/NF-κB pathway in HNECs and is implicated in eosinophil infiltration in patients with Eos CRSwNP.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:73 |
---|---|
Enthalten in: |
Inflammation research : official journal of the European Histamine Research Society ... [et al. - 73(2024), 4 vom: 22. März, Seite 499-513 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Huang, Zizhen [VerfasserIn] |
---|
Links: |
---|
Themen: |
Chronic rhinosinusitis with nasal polyps |
---|
Anmerkungen: |
Date Completed 29.03.2024 Date Revised 29.03.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1007/s00011-024-01851-2 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM368833291 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM368833291 | ||
003 | DE-627 | ||
005 | 20240330001015.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240229s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1007/s00011-024-01851-2 |2 doi | |
028 | 5 | 2 | |a pubmed24n1355.xml |
035 | |a (DE-627)NLM368833291 | ||
035 | |a (NLM)38393344 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Huang, Zizhen |e verfasserin |4 aut | |
245 | 1 | 0 | |a Interleukin-19 enhances eosinophil infiltration through upregulation of epithelium-derived RANTES expression via the ERK/NF-κB signalling pathway in patients with eosinophilic CRSwNP |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 29.03.2024 | ||
500 | |a Date Revised 29.03.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG. | ||
520 | |a BACKGROUD: The recurrence rate of chronic rhinosinusitis with nasal polyps (CRSwNP) is positively correlated with eosinophil infiltration. Increased interleukin (IL)-19 and eosinophil chemokine RANTES levels have been reported in patients with CRSwNP. This study aimed to clarify the role of IL-19 in mediating RANTES expression and eosinophilic infiltration in eosinophilic CRSwNP (Eos CRSwNP) | ||
520 | |a METHODS: Nasal tissue samples were obtained from patients with CRSwNP and controls. The expression of IL-19, its receptors, ECP, and RANTES in tissues was investigated. Primary human nasal epithelial cells (HNECs) and nasal polyp tissue blocks were cultured, then stimulated by IL-19; ERK phosphorylation, NF-κB pathway activation, RANTES level, eosinophils migration and infiltration were detected using RT-qPCR, ELISA, western blotting, HE, immunohistochemistry, immunofluorescence staining, confocal microscopy, and transwell migration assay | ||
520 | |a RESULTS: The expression of IL-19 and its receptors (IL-20R1/IL-20R2), eosinophil cationic protein, and RANTES in nasal tissues from patients with Eos CRSwNP was significantly increased compared to that in non-Eos CRSwNP and control subjects. IL-19 co-localized with RANTES in nasal tissues and significantly elevated RANTES expression in HNECs. IL-19-blocking antibody and siRNA knockdown of IL-20R1 ameliorated the effect of IL-19 on RANTES secretion in HNECs. Moreover, IL-19-induced RANTES upregulation was associated with the activation of the ERK and NF-κB pathways. NF-κB activation was mediated by the ERK pathway in IL-19-treated HNECs, and IL-19 enhanced eosinophil infiltration in nasal polyp tissue blocks | ||
520 | |a CONCLUSIONS: Our findings indicate that IL-19 promotes RANTES expression via the ERK/NF-κB pathway in HNECs and is implicated in eosinophil infiltration in patients with Eos CRSwNP | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Chronic rhinosinusitis with nasal polyps | |
650 | 4 | |a Eosinophil | |
650 | 4 | |a IL-19 | |
650 | 4 | |a Nasal epithelium | |
650 | 4 | |a RANTES | |
650 | 7 | |a NF-kappa B |2 NLM | |
650 | 7 | |a Interleukins |2 NLM | |
700 | 1 | |a Li, Xia |e verfasserin |4 aut | |
700 | 1 | |a Li, Yue |e verfasserin |4 aut | |
700 | 1 | |a Huang, Weiqiang |e verfasserin |4 aut | |
700 | 1 | |a Lai, Xiaoping |e verfasserin |4 aut | |
700 | 1 | |a Wu, Haotian |e verfasserin |4 aut | |
700 | 1 | |a Chen, Xiaohong |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Yana |e verfasserin |4 aut | |
700 | 1 | |a Chang, Lihong |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Gehua |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Inflammation research : official journal of the European Histamine Research Society ... [et al. |d 1997 |g 73(2024), 4 vom: 22. März, Seite 499-513 |w (DE-627)NLM075208601 |x 1420-908X |7 nnns |
773 | 1 | 8 | |g volume:73 |g year:2024 |g number:4 |g day:22 |g month:03 |g pages:499-513 |
856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00011-024-01851-2 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 73 |j 2024 |e 4 |b 22 |c 03 |h 499-513 |