PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay : A highly sensitive method for detection of MYD88 L265P mutation
© 2024 John Wiley & Sons Ltd..
INTRODUCTION: Agarose gel-based conventional and real-time allele-specific polymerase chain reaction (AS-PCR) assays are currently used for sensitive detection and quantification of MYD88 L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele-specific quantification PCR (AS-qPCR) assay, PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay, that uses Intercalating Nucleic Acid (INA®) technology for increased affinity and specificity.
METHODS: This study compares PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay with conventional AS-PCR. We included a total of 102 peripheral and bone marrow blood samples from 94 patients with a lymphoproliferative disorder. Droplet digital PCR (ddPCR) was used as a third method in case of discrepancy.
RESULTS: A positive percent agreement of 100% (95% CI 0.92-1.0) and a negative percent agreement of 98% (95% CI 0.90-1.0) were found between the conventional AS-PCR and the AS-qPCR methods. Including the ddPCR results to validate the discrepant cases, the sensitivity and specificity of PlentiPlex™ MYD88 Waldenström lymphoma qPCR Assay were 1.0 (95% CI 0.97-1.0) and 1.0 (95% CI 0.96-1.0), respectively.
CONCLUSION: Our data demonstrate that PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay is a fast, highly sensitive, and specific method for the detection of MYD88 L265P compared with conventional AS-PCR.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
International journal of laboratory hematology - (2024) vom: 23. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Viscovo, Marcello [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 23.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1111/ijlh.14255 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368807908 |
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| 245 | 1 | 0 | |a PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay |b A highly sensitive method for detection of MYD88 L265P mutation |
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| 520 | |a © 2024 John Wiley & Sons Ltd. | ||
| 520 | |a INTRODUCTION: Agarose gel-based conventional and real-time allele-specific polymerase chain reaction (AS-PCR) assays are currently used for sensitive detection and quantification of MYD88 L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele-specific quantification PCR (AS-qPCR) assay, PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay, that uses Intercalating Nucleic Acid (INA®) technology for increased affinity and specificity | ||
| 520 | |a METHODS: This study compares PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay with conventional AS-PCR. We included a total of 102 peripheral and bone marrow blood samples from 94 patients with a lymphoproliferative disorder. Droplet digital PCR (ddPCR) was used as a third method in case of discrepancy | ||
| 520 | |a RESULTS: A positive percent agreement of 100% (95% CI 0.92-1.0) and a negative percent agreement of 98% (95% CI 0.90-1.0) were found between the conventional AS-PCR and the AS-qPCR methods. Including the ddPCR results to validate the discrepant cases, the sensitivity and specificity of PlentiPlex™ MYD88 Waldenström lymphoma qPCR Assay were 1.0 (95% CI 0.97-1.0) and 1.0 (95% CI 0.96-1.0), respectively | ||
| 520 | |a CONCLUSION: Our data demonstrate that PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay is a fast, highly sensitive, and specific method for the detection of MYD88 L265P compared with conventional AS-PCR | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a IgM monoclonal gammopathy of unknown significance (MGUS) | |
| 650 | 4 | |a Waldenström macroglobulinemia (WM) | |
| 650 | 4 | |a allele-specific polymerase chain reaction (AS-PCR) | |
| 650 | 4 | |a chronic lymphocytic leukemia (CLL) | |
| 650 | 4 | |a droplet digital PCR (ddPCR) | |
| 650 | 4 | |a lymphoplasmacytic lymphoma (LPL) | |
| 650 | 4 | |a marginal zone lymphoma (MZL) | |
| 650 | 4 | |a multiple myeloma (MM) | |
| 650 | 4 | |a myeloid differentiation primary response 88 (MYD88) | |
| 700 | 1 | |a Clemmensen, Mia de Laurent |e verfasserin |4 aut | |
| 700 | 1 | |a Fosso, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Maiolo, Elena |e verfasserin |4 aut | |
| 700 | 1 | |a Autore, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Laurenti, Luca |e verfasserin |4 aut | |
| 700 | 1 | |a Hohaus, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Chiusolo, Patrizia |e verfasserin |4 aut | |
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