Prednisone and prednisolone effects on development, blood, biochemical and histopathological markers of Aquarana catesbeianus tadpoles
Copyright © 2024. Published by Elsevier B.V..
Synthetic glucocorticoids are often found in surface waters and can cause harmful effects to aquatic organisms such as amphibians. In this work we evaluated the effects of the drugs prednisone (PD) and prednisolone (PL) on developmental, molecular, blood, biochemical and histological markers. Aquarana catesbeianus tadpoles were exposed for 16 days to environmentally relevant concentrations of 0, 0.1, 1 and 10 µg/L of both drugs. PD increased the transcript levels of the enzyme deiodinase III (Dio3), the hormones cortisol and T4 and delayed development. Changes in the thyroid gland occurred after tadpoles were exposed to both drugs, with a reduction in the diameter and number of follicles and an increase/or decrease in area. Also, both drugs caused a decrease in lymphocytes (L) and an increase in neutrophils (N), thrombocytes, the N:L ratio and lobed and notched erythrocytes. Increased activity of the enzymes superoxide dismutase, glutathione S-transferase and glucose 6-phosphate dehydrogenase was observed after exposure to PD. Furthermore, both drugs caused an increase in the activity of the enzymes catalase and glutathione peroxidase. However, only PD caused oxidative stress in exposed tadpoles, evidenced by increased levels of malondialdehyde and carbonyl proteins. Both drugs caused an increase in inflammatory infiltrates, blood cells and melanomacrophages in the liver. Our results indicate that PD was more toxic than PL, affecting development and causing oxidative stress.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:268 |
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| Enthalten in: |
Aquatic toxicology (Amsterdam, Netherlands) - 268(2024) vom: 21. März, Seite 106869 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rutkoski, Camila Fatima [VerfasserIn] |
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| Links: |
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| Themen: |
9PHQ9Y1OLM |
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| Anmerkungen: |
Date Completed 01.03.2024 Date Revised 01.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.aquatox.2024.106869 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368772365 |
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| 520 | |a Copyright © 2024. Published by Elsevier B.V. | ||
| 520 | |a Synthetic glucocorticoids are often found in surface waters and can cause harmful effects to aquatic organisms such as amphibians. In this work we evaluated the effects of the drugs prednisone (PD) and prednisolone (PL) on developmental, molecular, blood, biochemical and histological markers. Aquarana catesbeianus tadpoles were exposed for 16 days to environmentally relevant concentrations of 0, 0.1, 1 and 10 µg/L of both drugs. PD increased the transcript levels of the enzyme deiodinase III (Dio3), the hormones cortisol and T4 and delayed development. Changes in the thyroid gland occurred after tadpoles were exposed to both drugs, with a reduction in the diameter and number of follicles and an increase/or decrease in area. Also, both drugs caused a decrease in lymphocytes (L) and an increase in neutrophils (N), thrombocytes, the N:L ratio and lobed and notched erythrocytes. Increased activity of the enzymes superoxide dismutase, glutathione S-transferase and glucose 6-phosphate dehydrogenase was observed after exposure to PD. Furthermore, both drugs caused an increase in the activity of the enzymes catalase and glutathione peroxidase. However, only PD caused oxidative stress in exposed tadpoles, evidenced by increased levels of malondialdehyde and carbonyl proteins. Both drugs caused an increase in inflammatory infiltrates, blood cells and melanomacrophages in the liver. Our results indicate that PD was more toxic than PL, affecting development and causing oxidative stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aquatic toxicology | |
| 650 | 4 | |a Corticoid | |
| 650 | 4 | |a Emerging contaminants | |
| 650 | 4 | |a Pollutant biomarkers | |
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| 700 | 1 | |a Grott, Suelen Cristina |e verfasserin |4 aut | |
| 700 | 1 | |a Israel, Nicole Grasmuk |e verfasserin |4 aut | |
| 700 | 1 | |a Guerreiro, Fernando de Campos |e verfasserin |4 aut | |
| 700 | 1 | |a Carneiro, Francisco Estevão |e verfasserin |4 aut | |
| 700 | 1 | |a Bitschinski, Daiane |e verfasserin |4 aut | |
| 700 | 1 | |a Warsneski, Aline |e verfasserin |4 aut | |
| 700 | 1 | |a Horn, Priscila Aparecida |e verfasserin |4 aut | |
| 700 | 1 | |a Lima, Daína |e verfasserin |4 aut | |
| 700 | 1 | |a Bastolla, Camila Lisarb Velasquez |e verfasserin |4 aut | |
| 700 | 1 | |a Mattos, Jacó Joaquim |e verfasserin |4 aut | |
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| 700 | 1 | |a da Silva, Elizia Barbosa |e verfasserin |4 aut | |
| 700 | 1 | |a de Albuquerque, Claudia Almeida Coelho |e verfasserin |4 aut | |
| 700 | 1 | |a Alves, Thiago Caique |e verfasserin |4 aut | |
| 700 | 1 | |a de Almeida, Eduardo Alves |e verfasserin |4 aut | |
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