Aberrant miR-29 is a predictive feature of severe phenotypes in pediatric Crohn's disease
Crohn's disease (CD) is a chronic inflammatory gut disorder. Molecular mechanisms underlying the clinical heterogeneity of CD remain poorly understood. MicroRNAs (miRNAs) are important regulators of gut physiology, and several have been implicated in the pathogenesis of adult CD. However, there is a dearth of large-scale miRNA studies for pediatric CD. We hypothesized that specific miRNAs uniquely mark pediatric CD. We performed small RNA-Seq of patient-matched colon and ileum biopsies from treatment-naive pediatric patients with CD (n = 169) and a control cohort (n = 108). Comprehensive miRNA analysis revealed 58 miRNAs altered in pediatric CD. Notably, multinomial logistic regression analysis revealed that index levels of ileal miR-29 are strongly predictive of severe inflammation and stricturing. Transcriptomic analyses of transgenic mice overexpressing miR-29 show a significant reduction of the tight junction protein gene Pmp22 and classic Paneth cell markers. The dramatic loss of Paneth cells was confirmed by histologic assays. Moreover, we found that pediatric patients with CD with elevated miR-29 exhibit significantly lower Paneth cell counts, increased inflammation scores, and reduced levels of PMP22. These findings strongly indicate that miR-29 upregulation is a distinguishing feature of pediatric CD, highly predictive of severe phenotypes, and associated with inflammation and Paneth cell loss.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
JCI insight - 9(2024), 4 vom: 22. Feb. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Shumway, Alexandria J [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Gastroenterology |
|---|
| Anmerkungen: |
Date Completed 23.02.2024 Date Revised 30.03.2024 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1172/jci.insight.168800 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM36875748X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM36875748X | ||
| 003 | DE-627 | ||
| 005 | 20240331001001.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240222s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1172/jci.insight.168800 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1357.xml |
| 035 | |a (DE-627)NLM36875748X | ||
| 035 | |a (NLM)38385744 | ||
| 035 | |a (PII)e168800 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Shumway, Alexandria J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Aberrant miR-29 is a predictive feature of severe phenotypes in pediatric Crohn's disease |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.02.2024 | ||
| 500 | |a Date Revised 30.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Crohn's disease (CD) is a chronic inflammatory gut disorder. Molecular mechanisms underlying the clinical heterogeneity of CD remain poorly understood. MicroRNAs (miRNAs) are important regulators of gut physiology, and several have been implicated in the pathogenesis of adult CD. However, there is a dearth of large-scale miRNA studies for pediatric CD. We hypothesized that specific miRNAs uniquely mark pediatric CD. We performed small RNA-Seq of patient-matched colon and ileum biopsies from treatment-naive pediatric patients with CD (n = 169) and a control cohort (n = 108). Comprehensive miRNA analysis revealed 58 miRNAs altered in pediatric CD. Notably, multinomial logistic regression analysis revealed that index levels of ileal miR-29 are strongly predictive of severe inflammation and stricturing. Transcriptomic analyses of transgenic mice overexpressing miR-29 show a significant reduction of the tight junction protein gene Pmp22 and classic Paneth cell markers. The dramatic loss of Paneth cells was confirmed by histologic assays. Moreover, we found that pediatric patients with CD with elevated miR-29 exhibit significantly lower Paneth cell counts, increased inflammation scores, and reduced levels of PMP22. These findings strongly indicate that miR-29 upregulation is a distinguishing feature of pediatric CD, highly predictive of severe phenotypes, and associated with inflammation and Paneth cell loss | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gastroenterology | |
| 650 | 4 | |a Genetics | |
| 650 | 4 | |a Inflammatory bowel disease | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a MIRN29 microRNA, mouse |2 NLM | |
| 700 | 1 | |a Shanahan, Michael T |e verfasserin |4 aut | |
| 700 | 1 | |a Hollville, Emilie |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Kevin |e verfasserin |4 aut | |
| 700 | 1 | |a Beasley, Caroline |e verfasserin |4 aut | |
| 700 | 1 | |a Villanueva, Jonathan W |e verfasserin |4 aut | |
| 700 | 1 | |a Albert, Sara |e verfasserin |4 aut | |
| 700 | 1 | |a Lian, Grace |e verfasserin |4 aut | |
| 700 | 1 | |a Cure, Moises R |e verfasserin |4 aut | |
| 700 | 1 | |a Schaner, Matthew |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Lee-Ching |e verfasserin |4 aut | |
| 700 | 1 | |a Bantumilli, Surekha |e verfasserin |4 aut | |
| 700 | 1 | |a Deshmukh, Mohanish |e verfasserin |4 aut | |
| 700 | 1 | |a Furey, Terrence S |e verfasserin |4 aut | |
| 700 | 1 | |a Sheikh, Shehzad Z |e verfasserin |4 aut | |
| 700 | 1 | |a Sethupathy, Praveen |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t JCI insight |d 2016 |g 9(2024), 4 vom: 22. Feb. |w (DE-627)NLM257703918 |x 2379-3708 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2024 |g number:4 |g day:22 |g month:02 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1172/jci.insight.168800 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2024 |e 4 |b 22 |c 02 | ||