Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil
Sporothrix brasiliensis is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61 Sporothrix spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of S. brasiliensis isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the in vitro resistant isolates were not clonal and were distributed across three different S. brasiliensis clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of in vitro resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene, cyp51, corresponding to the well-known G448S substitution inducing azole resistance in Aspergillus fumigatus. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26 in vitro resistant isolates.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
|---|---|
| Enthalten in: |
Antimicrobial agents and chemotherapy - 68(2024), 4 vom: 03. Apr., Seite e0162023 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ribeiro Dos Santos, Amanda [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
304NUG5GF4 |
|---|
| Anmerkungen: |
Date Completed 04.04.2024 Date Revised 05.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1128/aac.01620-23 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM368757064 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM368757064 | ||
| 003 | DE-627 | ||
| 005 | 20240405233617.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240222s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1128/aac.01620-23 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1366.xml |
| 035 | |a (DE-627)NLM368757064 | ||
| 035 | |a (NLM)38385701 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ribeiro Dos Santos, Amanda |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.04.2024 | ||
| 500 | |a Date Revised 05.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Sporothrix brasiliensis is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61 Sporothrix spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of S. brasiliensis isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the in vitro resistant isolates were not clonal and were distributed across three different S. brasiliensis clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of in vitro resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene, cyp51, corresponding to the well-known G448S substitution inducing azole resistance in Aspergillus fumigatus. SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26 in vitro resistant isolates | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a antifungal agents | |
| 650 | 4 | |a antifungal resistance | |
| 650 | 4 | |a sporotrichosis | |
| 650 | 7 | |a Antifungal Agents |2 NLM | |
| 650 | 7 | |a Azoles |2 NLM | |
| 650 | 7 | |a Itraconazole |2 NLM | |
| 650 | 7 | |a 304NUG5GF4 |2 NLM | |
| 700 | 1 | |a Gade, Lalitha |e verfasserin |4 aut | |
| 700 | 1 | |a Misas, Elizabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Litvintseva, Anastasia P |e verfasserin |4 aut | |
| 700 | 1 | |a Nunnally, Natalie S |e verfasserin |4 aut | |
| 700 | 1 | |a Parnell, Lindsay A |e verfasserin |4 aut | |
| 700 | 1 | |a Rajeev, Malavika |e verfasserin |4 aut | |
| 700 | 1 | |a de Souza Carvalho Melhem, Marcia |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Juliana Possato Fernandes |e verfasserin |4 aut | |
| 700 | 1 | |a Oliboni, Gabriel Manzi |e verfasserin |4 aut | |
| 700 | 1 | |a Bonfieti, Lucas Xavier |e verfasserin |4 aut | |
| 700 | 1 | |a Araujo, Lisandra Siufi |e verfasserin |4 aut | |
| 700 | 1 | |a Cappellano, Paola |e verfasserin |4 aut | |
| 700 | 1 | |a Venturini, James |e verfasserin |4 aut | |
| 700 | 1 | |a Lockhart, Shawn R |e verfasserin |4 aut | |
| 700 | 1 | |a Sexton, D Joseph |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Antimicrobial agents and chemotherapy |d 1972 |g 68(2024), 4 vom: 03. Apr., Seite e0162023 |w (DE-627)NLM000026506 |x 1098-6596 |7 nnns |
| 773 | 1 | 8 | |g volume:68 |g year:2024 |g number:4 |g day:03 |g month:04 |g pages:e0162023 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1128/aac.01620-23 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 68 |j 2024 |e 4 |b 03 |c 04 |h e0162023 | ||