The genome of Stephania japonica provides insights into the biosynthesis of cepharanthine
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved..
Stephania japonica is an early-diverging eudicotyledon plant with high levels of cepharanthine, proven to be effective in curing coronavirus infections. Here, we report a high-quality S. japonica genome. The genome size is 688.52 Mb, and 97.37% sequences anchor to 11 chromosomes. The genome comprises 67.46% repetitive sequences and 21,036 genes. It is closely related to two Ranunculaceae species, which diverged from their common ancestor 55.90-71.02 million years ago (Mya) with a whole-genome duplication 85.59-96.75 Mya. We further reconstruct ancestral karyotype of Ranunculales. Several cepharanthine biosynthesis genes are identified and verified by western blot. Two genes (Sja03G0243 and Sja03G0241) exhibit catalytic activity as shown by liquid chromatography-mass spectrometry. Then, cepharanthine biosynthesis genes, transcription factors, and CYP450 family genes are used to construct a comprehensive network. Finally, we construct an early-diverging eudicotyledonous genome resources (EEGR) database. As the first genome of the Menispermaceae family to be released, this study provides rich resources for genomic studies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Cell reports - 43(2024), 3 vom: 26. März, Seite 113832 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Zhuo [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.04.2024 Date Revised 01.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2024.113832 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 4 | |a The genome of Stephania japonica provides insights into the biosynthesis of cepharanthine |
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| 520 | |a Stephania japonica is an early-diverging eudicotyledon plant with high levels of cepharanthine, proven to be effective in curing coronavirus infections. Here, we report a high-quality S. japonica genome. The genome size is 688.52 Mb, and 97.37% sequences anchor to 11 chromosomes. The genome comprises 67.46% repetitive sequences and 21,036 genes. It is closely related to two Ranunculaceae species, which diverged from their common ancestor 55.90-71.02 million years ago (Mya) with a whole-genome duplication 85.59-96.75 Mya. We further reconstruct ancestral karyotype of Ranunculales. Several cepharanthine biosynthesis genes are identified and verified by western blot. Two genes (Sja03G0243 and Sja03G0241) exhibit catalytic activity as shown by liquid chromatography-mass spectrometry. Then, cepharanthine biosynthesis genes, transcription factors, and CYP450 family genes are used to construct a comprehensive network. Finally, we construct an early-diverging eudicotyledonous genome resources (EEGR) database. As the first genome of the Menispermaceae family to be released, this study provides rich resources for genomic studies | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a CP: Genomics | |
| 650 | 4 | |a CP: Plants | |
| 650 | 4 | |a S. japonica | |
| 650 | 4 | |a benzylisoquinoline alkaloid | |
| 650 | 4 | |a cepharanthine | |
| 650 | 4 | |a database construction | |
| 650 | 4 | |a genome assembly and evolution | |
| 650 | 4 | |a karyotype reconstruction | |
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| 700 | 1 | |a Shen, Shaoqin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yujie |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Shuqi |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Tong |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Yanhong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chunjin |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yanshu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Liangdan |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Xiaoming |e verfasserin |4 aut | |
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