An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples
© 2024 The Authors..
Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristics:•The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples.•A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time.•Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 %.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
MethodsX - 12(2024) vom: 19. Feb., Seite 102596 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Appeltrath, Gwenllian A [VerfasserIn] |
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| Links: |
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| Themen: |
ELISA |
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| Anmerkungen: |
Date Revised 22.02.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.mex.2024.102596 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368697363 |
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| 245 | 1 | 3 | |a An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples |
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| 520 | |a © 2024 The Authors. | ||
| 520 | |a Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristics:•The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples.•A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time.•Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 % | ||
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| 700 | 1 | |a Lindemann, Monika |e verfasserin |4 aut | |
| 700 | 1 | |a Klump, Hannes |e verfasserin |4 aut | |
| 700 | 1 | |a Karsten, Christina B |e verfasserin |4 aut | |
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