Real-time genomic epidemiologic investigation of a multispecies plasmid-associated hospital outbreak of NDM-5-producing Enterobacterales infections
Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved..
OBJECTIVES: New Delhi metallo-β-lactamase (NDM) is an emergent mechanism of carbapenem resistance associated with high mortality and limited treatment options. Because the blaNDM resistance gene is often carried on plasmids, traditional infection prevention and control (IP&C) surveillance methods and reactive whole genome sequencing (WGS) may not detect plasmid transfer in multispecies outbreaks.
METHODS: Initial outbreak detection of NDM-producing Enterobacterales identified at an acute care hospital occurred via traditional IP&C methods and was supplemented by real-time WGS surveillance performed weekly. To resolve NDM-encoding plasmids, we performed long-read sequencing and constructed hybrid assemblies. WGS data for suspected outbreaks was shared with the IP&C team for assessment and intervention.
RESULTS: We observed a multispecies outbreak of NDM-5-producing Enterobacterales isolated from 15 patients between February 2021 and February 2023. The 19 clinical and surveillance isolates sequenced included 7 bacterial species encoding the same NDM-5 plasmid. WGS surveillance and epidemiologic investigation characterized 10 horizontal plasmid transfer events and 6 bacterial transmission events between patients in varying hospital units.
CONCLUSIONS: Our investigation revealed a complex, multispecies outbreak of NDM involving multiple plasmid transfer and bacterial transmission events. We highlight the utility of combining traditional IP&C and prospective genomic methods in identifying and containing plasmid-associated outbreaks.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:142 |
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| Enthalten in: |
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 142(2024) vom: 19. Apr., Seite 106971 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Raabe, Nathan J [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-Bacterial Agents |
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| Anmerkungen: |
Date Completed 16.04.2024 Date Revised 28.04.2024 published: Print-Electronic UpdateOf: medRxiv. 2023 Sep 01;:. - PMID 37693518 Citation Status MEDLINE |
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| doi: |
10.1016/j.ijid.2024.02.014 |
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| PPN (Katalog-ID): |
NLM368637115 |
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| 100 | 1 | |a Raabe, Nathan J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Real-time genomic epidemiologic investigation of a multispecies plasmid-associated hospital outbreak of NDM-5-producing Enterobacterales infections |
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| 500 | |a Date Revised 28.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a UpdateOf: medRxiv. 2023 Sep 01;:. - PMID 37693518 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a OBJECTIVES: New Delhi metallo-β-lactamase (NDM) is an emergent mechanism of carbapenem resistance associated with high mortality and limited treatment options. Because the blaNDM resistance gene is often carried on plasmids, traditional infection prevention and control (IP&C) surveillance methods and reactive whole genome sequencing (WGS) may not detect plasmid transfer in multispecies outbreaks | ||
| 520 | |a METHODS: Initial outbreak detection of NDM-producing Enterobacterales identified at an acute care hospital occurred via traditional IP&C methods and was supplemented by real-time WGS surveillance performed weekly. To resolve NDM-encoding plasmids, we performed long-read sequencing and constructed hybrid assemblies. WGS data for suspected outbreaks was shared with the IP&C team for assessment and intervention | ||
| 520 | |a RESULTS: We observed a multispecies outbreak of NDM-5-producing Enterobacterales isolated from 15 patients between February 2021 and February 2023. The 19 clinical and surveillance isolates sequenced included 7 bacterial species encoding the same NDM-5 plasmid. WGS surveillance and epidemiologic investigation characterized 10 horizontal plasmid transfer events and 6 bacterial transmission events between patients in varying hospital units | ||
| 520 | |a CONCLUSIONS: Our investigation revealed a complex, multispecies outbreak of NDM involving multiple plasmid transfer and bacterial transmission events. We highlight the utility of combining traditional IP&C and prospective genomic methods in identifying and containing plasmid-associated outbreaks | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a New-Delhi metallo-β-lactamase | |
| 650 | 4 | |a Outbreak investigation | |
| 650 | 4 | |a Plasmid | |
| 650 | 4 | |a Whole genome sequencing | |
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| 700 | 1 | |a Valek, Abby L |e verfasserin |4 aut | |
| 700 | 1 | |a Griffith, Marissa P |e verfasserin |4 aut | |
| 700 | 1 | |a Mills, Emma |e verfasserin |4 aut | |
| 700 | 1 | |a Waggle, Kady |e verfasserin |4 aut | |
| 700 | 1 | |a Srinivasa, Vatsala Rangachar |e verfasserin |4 aut | |
| 700 | 1 | |a Ayres, Ashley M |e verfasserin |4 aut | |
| 700 | 1 | |a Bradford, Claire |e verfasserin |4 aut | |
| 700 | 1 | |a Creager, Hannah M |e verfasserin |4 aut | |
| 700 | 1 | |a Pless, Lora L |e verfasserin |4 aut | |
| 700 | 1 | |a Sundermann, Alexander J |e verfasserin |4 aut | |
| 700 | 1 | |a Van Tyne, Daria |e verfasserin |4 aut | |
| 700 | 1 | |a Snyder, Graham M |e verfasserin |4 aut | |
| 700 | 1 | |a Harrison, Lee H |e verfasserin |4 aut | |
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