Vitamin D3 mitigates type 2 diabetes induced by a high carbohydrate-high fat diet in rats : Role of the purinergic system
Copyright © 2024 Elsevier Inc. All rights reserved..
This study evaluated the effect of vitamin D3 (VIT D3) supplementation on the enzymatic activities and density of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5-nucleotidase (E-5'-NT), adenosine deaminase (ADA), as well as the density of P2 × 7R, P2Y12R, A1R, A2AR receptors, IL-1β, and oxidative parameters in type 2 diabetic rats. Forty male Wistar rats were fed a high carbohydrate-high fat diet (HCHFD) and received an intraperitoneal injection containing a single dose of streptozotocin (STZ, 35 mg/kg). Animals were divided into four groups: 1) control; 2) control/VIT D3 12 µg/kg; 3) diabetic; and 4) diabetic/VIT D3 12 µg/kg. Results show that VIT D3 reduced blood glucose, ATP hydrolysis, ADA activity, P2Y12R density (platelets), as well as ATP, ADP, and AMP hydrolysis and ADA activity (synaptosomes). Moreover, VIT D3 increased insulin levels and AMP hydrolysis (platelets) and improved antioxidant defense. Therefore, we suggest that VIT D3 treatment modulates hyperglycemia-induced changes via purinergic enzymes and receptor expression, consequently attenuating insulin homeostasis dysregulation in the diabetic state.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:127 |
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| Enthalten in: |
The Journal of nutritional biochemistry - 127(2024) vom: 15. Apr., Seite 109602 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vera Castro, Milagros Fanny [VerfasserIn] |
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| Links: |
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| Themen: |
1C6V77QF41 |
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| Anmerkungen: |
Date Completed 08.04.2024 Date Revised 08.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jnutbio.2024.109602 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368635740 |
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| 520 | |a This study evaluated the effect of vitamin D3 (VIT D3) supplementation on the enzymatic activities and density of ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-5-nucleotidase (E-5'-NT), adenosine deaminase (ADA), as well as the density of P2 × 7R, P2Y12R, A1R, A2AR receptors, IL-1β, and oxidative parameters in type 2 diabetic rats. Forty male Wistar rats were fed a high carbohydrate-high fat diet (HCHFD) and received an intraperitoneal injection containing a single dose of streptozotocin (STZ, 35 mg/kg). Animals were divided into four groups: 1) control; 2) control/VIT D3 12 µg/kg; 3) diabetic; and 4) diabetic/VIT D3 12 µg/kg. Results show that VIT D3 reduced blood glucose, ATP hydrolysis, ADA activity, P2Y12R density (platelets), as well as ATP, ADP, and AMP hydrolysis and ADA activity (synaptosomes). Moreover, VIT D3 increased insulin levels and AMP hydrolysis (platelets) and improved antioxidant defense. Therefore, we suggest that VIT D3 treatment modulates hyperglycemia-induced changes via purinergic enzymes and receptor expression, consequently attenuating insulin homeostasis dysregulation in the diabetic state | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Hyperglycemia | |
| 650 | 4 | |a Insulin | |
| 650 | 4 | |a Oxidative stress | |
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| 700 | 1 | |a Assmann, Charles Elias |e verfasserin |4 aut | |
| 700 | 1 | |a Reichert, Karine Paula |e verfasserin |4 aut | |
| 700 | 1 | |a Coppetti, Priscila Marquezan |e verfasserin |4 aut | |
| 700 | 1 | |a Stefanello, Naiara |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Aniélen Dutra |e verfasserin |4 aut | |
| 700 | 1 | |a Mostardeiro, Vitor Bastianello |e verfasserin |4 aut | |
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| 700 | 1 | |a Morsch, Vera Maria Melchiors |e verfasserin |4 aut | |
| 700 | 1 | |a Schetinger, Maria Rosa Chitolina |e verfasserin |4 aut | |
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