Mechanistic study of transcription factor Sox18 during heart development
Copyright © 2024 Elsevier Inc. All rights reserved..
Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:350 |
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Enthalten in: |
General and comparative endocrinology - 350(2024) vom: 01. März, Seite 114472 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Liang, Jianxin [VerfasserIn] |
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Links: |
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Themen: |
Beta Catenin |
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Anmerkungen: |
Date Completed 25.03.2024 Date Revised 27.03.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.ygcen.2024.114472 |
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funding: |
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PPN (Katalog-ID): |
NLM36863521X |
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520 | |a Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Heart development | |
650 | 4 | |a Sox18 | |
650 | 4 | |a Transcriptional factor | |
650 | 4 | |a Wnt signaling pathway | |
650 | 4 | |a Xenopus | |
650 | 7 | |a beta Catenin |2 NLM | |
650 | 7 | |a SOX18 protein, human |2 NLM | |
650 | 7 | |a SOXF Transcription Factors |2 NLM | |
650 | 7 | |a Xenopus Proteins |2 NLM | |
650 | 7 | |a sox18 protein, Xenopus |2 NLM | |
700 | 1 | |a Ul Hassan, Imtiaz |e verfasserin |4 aut | |
700 | 1 | |a Yee Cheung, Man |e verfasserin |4 aut | |
700 | 1 | |a Feng, Lei |e verfasserin |4 aut | |
700 | 1 | |a Lin, Yi-Jyun |e verfasserin |4 aut | |
700 | 1 | |a Long, Qi |e verfasserin |4 aut | |
700 | 1 | |a Wang, Chengdong |e verfasserin |4 aut | |
700 | 1 | |a Ding, Yuyue |e verfasserin |4 aut | |
700 | 1 | |a Wang, Ziqing |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Yuan |e verfasserin |4 aut | |
700 | 1 | |a Li, Yulong |e verfasserin |4 aut | |
700 | 1 | |a Guo, Donghao |e verfasserin |4 aut | |
700 | 1 | |a Guo, Xiaofang |e verfasserin |4 aut | |
700 | 1 | |a Chi Bun Wong, Thomas |e verfasserin |4 aut | |
700 | 1 | |a Kaleem Samma, Muhammad |e verfasserin |4 aut | |
700 | 1 | |a Rong, Zixin |e verfasserin |4 aut | |
700 | 1 | |a Qi, Xufeng |e verfasserin |4 aut | |
700 | 1 | |a Cai, Dongqing |e verfasserin |4 aut | |
700 | 1 | |a Ngai, Sai-Ming |e verfasserin |4 aut | |
700 | 1 | |a Zhao, Hui |e verfasserin |4 aut | |
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